Effect of Para Substituted Anilines Chemical Structure on Methane Biosynthesis by the Methanogens.
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Date
2013-10
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Abstract
The present paper aims to study the effect of aromatic structure on the inhibition of
biogas production and more specifically the effect of para substituted anilines functional
groups (chemical structure) on methane biosynthesis by the digested pig manure
methanogens. The objective of this study was also to examine the structure-toxicity
relationships of aromatic compounds to acetoclastic methanogens.
Study Design: Anaerobic digestion of pig manure, anaerobic toxicity essay, The effects of
functional group nature on inhibition of methane production by acetoclastic methanogens.
Correlation of the methanogenic toxicity (IC50) with aromatic compounds hydrophobicity
(logPoct).
Place and Duration of Study: Department of Chemistry, University of Kinshasa (DR
Congo), between August 2011 and May 2012.
Methodology: The toxicity to acetoclastic methanogenic bacteria was performed with the
standard method of serum bottles, digested pig manure was utilized as inoculums, acetate as substrate and the methane gas volume produced was measured by serum bottles liquid
displacement systems (Mariotte flask system).
Results: The obtained results indicate that relationships exist between para substituted
anilines functional groups nature (chemical structure) and their inhibitory effects on
methanogens. The toxicity of para bisubstituted anilines increases in the following order:
SO3 < OH < H < CH3 < Cl < NO2
From this sequence of increasing toxicity, it can be seen that the methanogenic toxicity
varies with the functional group nature which is in the para position of the main function.
Indeed, p-Nitroaniline and benzene with 45.76 and 208.78 mg/l as IC50 values respectively
were the most toxic compounds, while p-Aminophenol and p-Aminosulfonic acid (Sulfanilic
acid) with 1800.39 and 2777.82 mg/l IC50 values were the less toxic.
A very significant negative linear correlation between the toxicity of para substituted
anilines compounds and their hydrophobicity was found.
Conclusion: The results of this study indicate that relationships exist between para
substituted anilines functional groups nature and their inhibitory effects in methane
biosynthesis by the methanogens.
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Keywords
Para substituted anilines, methane biosynthesis, chemical structure, toxicity, methanogens, digested pig manure
Citation
Kayembe K, Basosila L, Kalala K, Kitambala A K, Sikulisimwa C P, Mpiana P T. Effect of Para Substituted Anilines Chemical Structure on Methane Biosynthesis by the Methanogens. International Journal of Biochemistry Research & Review 2013 Oct-Dec ; 3 (4) : 303-314.