Cardiac Tolerance Study of Morinda morindoides (Baker) Milne-Redhead (Rubiaceae) Extract in Rabbit.
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Date
2013-10
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Abstract
This study was conducted to assess the effect of the ethyl acetate fraction
(AcEF1) obtained from the 96% extract of Morinda morindoides extract on cardiac tissue
integrity and function in rabbits.
Methodology: The rabbits were divided in five groups of 6 rabbits each. Groups 2,3,4 and
5 received intraperitoneally, twice a week during four weeks, the ethyl acetate fraction at
doses going from 25 to 100 μg/kg body weight while Group 1, received 1 mL of Mac Ewen
fluid. Blood sampling was carried out to evaluate aspartate aminotransferase (AST),
alanine aminotransferase (ALT), Creatine phosphokinase (CPK), Lactate dehydrogenase
(LDH) activities and level of calcium, sodium, potassium, magnesium and chloride in
serum. The eighth week of the experiments, histopathological studies were also conducted
on rabbits heart. Results: Analysis of the serum markers showed slight increases in AST, ALT, CPK
activities as well as sodium, calcium and potassium concentrations (p<0.05). LDH activity,
magnesium and chloride concentrations were unchanged compared to their initial values.
Histopathological studies had not revealed damages in the structure of the heart.
Conclusion: The ethyl acetate fraction did not exert any noxious effect on cardiac tissues
and should be no toxic for cardiac tissues.
Description
Keywords
Biochemical markers, Cardiac tolerance, Histopathology, Morinda morindoides
Citation
Tra-Bi Irie Otis, Djyh Bernard Nazaire, Coulibaly Amadou Founzegue, Djaman Allico Joseph, N’guessan Jean David. Cardiac Tolerance Study of Morinda morindoides (Baker) Milne-Redhead (Rubiaceae) Extract in Rabbit. International Journal of Biochemistry Research & Review 2013 Oct-Dec ; 3 (4) : 291-302.