Anti – Atherogenic Activity of Ethanol Extract of Buccholzia coriacea Seeds on Hypercholesterolemic Rats.
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Date
2013-07
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Abstract
To scientific determine the phytochemical constituents of ethanol extract of B.
coriacea (EEBC) powdered seeds and its effects on the serum lipid profile, serum
atherogenic index, and lipid peroxidation in the serum of hypercholesterolemic rats.
Study Design: Twenty male albino rats were used for the experiment. They were divided
into four groups, 5 rats in each. The administration is as follows: GROUP 1 (Normal
control): 0.3ml of corn oil only, GROUP 2 (experimental control): 40 mg/kg body weight
of cholesterol only, GROUP 3 (standard drug treatment): 40 mg/kg body weight of
cholesterol and 260mg/kg body weight of cholestyramine, GROUP 4 (extract treatment):
40 mg/kg body weight of cholesterol and 250mg/kg body weight of EEBC.
Place and Duration of Study: Department of Biochemistry, University of Ibadan, Nigeria
for eight weeks.
Methodology: At the end the 6 weeks of administration, the animals were kept fasting
overnight. They were then made unconscious by cervical dislocation and dissected. The
blood was then collected into clean vials. The Lipid profile, malondialdehdye levels were
measured in the serum of the rats in the four groups while the atherogenic index was
calculated. The qualitative phytochemical screening of EEBC seeds powder was performed. Data were statistically analyzed by one way ANOVA followed by post hoc
Duncan multiple range comparison tests.
Results: EEBC powdered seeds contains tannins, carbohydrates, saponins, flavonoids,
saponins, terpenoids and alkaloids. The level of serum total cholesterol, triglyceride, LDL –
cholesterol, atherogenic index significantly decreased (P=.05) by 29.5%, 41.5%, 39.2%,
77.4% respectively in the extract – treated group compared with experimental group.
Whereas the experimental group showed a significant (P=.05) increase compared with
normal control. There was a significant (P=.05) increase of 67.9% in the serum HDL -
cholesterol of the extract – treated group compared with the experimental group. Whereas
the experimental group showed a significant (P=.05) decrease in comparison with the
normal control. A significant (P=.05) decrease of 36% in the amount of serum
malondialdehyde was produced in the extract – treated groups compared with the
experimental control which showed a significant (P=.05) increase compared with the
normal control.
Conclusion: EEBC seed powder has potent anti- atherogenic properties.
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Keywords
Ethanol extract of Buccholzia coriacea (EEBC), hypercholesterolemia, cholestyramine, atherogenic index, lipid profile, lipid peroxidation
Citation
Omolekan T O, Olaiya C O O. Anti – Atherogenic Activity of Ethanol Extract of Buccholzia coriacea Seeds on Hypercholesterolemic Rats. International Journal of Biochemistry Research & Review 2013 Jul-Sept ; 3 (3) : 190-199.