Characterization of an Avian Protease Inhibitor.
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Date
2011-10
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Abstract
Ovomucoid is a serine proteinase inhibitor in the egg whites of all avian
species at a concentration of about 10 mg/ml. The involvement of proteinases in a
multitude of control functions in an organism has created an interest in their physiological
inhibitors. Regulation of proteolytic activity in tissues is a critical requirement in the
maintenance of homeostasis. Egg white proteins possess ACE-inhibitory activity, & also
high radical-scavenging activity. The combined antioxidant and ACE-inhibitory properties
of egg white hydrolysates, or the corresponding peptides, would make a useful
multifunctional preparation for the control of cardiovascular diseases. Proteases play key
roles in several physiological processes, including intracellular protein degradation, bone
remodeling, and antigen presentation, and their activities are increased in
pathophysiological conditions such as cancer metastasis and inflammation. They are
also required for invasion by microorganism. Four protease inhibitors have been
identified in egg white: cystatin, ovomucoid, ovomacroglobulin (also known as
ovostatin), and ovoinhibitor. Use of proteinase inhibitors in the treatment of certain
diseases has renewed interest in their specificity and stability, both of which in turn
depend on the tertiary structure of the inhibitor. Structural alteration to obtain molecules
of desired properties requires knowledge of relationship between structure, function and
stability.
Aims: In view of its importance, in the present study duck ovomucoid was isolated and
characterized for its physicochemical properties.
Methodology: Duck ovomucoid was isolated and characterized for its physicochemical
properties. Analytical gel filtration (Sephacryl S-100 HR column) was used for
purification, determination of molecular weight (MW), carbohydrate content and Stokes
radius.
Description
Keywords
Avian proteinase inhibitor, ovomucoid, physicochemical properties
Citation
Salman Khushtar Anwar, Ahmed Sharique, Saxena Indu. Characterization of an Avian Protease Inhibitor. International Journal of Biochemistry Research & Review 2011 Oct-Dec ; 1(3) : 58-68.