Study of photodynamic, sonodynamic and antioxidative influence on HeLa cell line.
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Date
2014-02
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Abstract
Photodynamic treatment (PDT) in combination with sonodynamic treatment (SDT) can be used as suitable methods to treat malignant and benign diseases or combat resistant bacteria. Both methods affect the production of reactive oxygen species (ROS). On the other hand, antioxidants are useful for cell protection against ROS. This work was aimed to study the effect of PDT and SDT treatments on the HeLa cell line using antioxidant Pronalen Sensitive SkinĀ® as a protection from free radicals in the cells. We evaluated the effect of sensitizer ClAlPcS2 using battery of in vitro methods, including MTT assay, kinetic production of ROS, mitochondrial membrane potential change, type of cell death and microscopic analysis. Ultrasound treatment was observed to increase the production of ROS, only in combination with PDT, particularly at higher concentrations of ClAlPcS2. The added antioxidant acts as protection against free radicals and has potential as a dietary supplement against aging or free radicals. The results of study suggested that ClAlPcS2 could be used as a potential photosensitizer for treatment of a specific type of cancers.
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Keywords
Photodynamic treatment, Sonodynamic treatment, Antioxidant, Reactive oxygen species, Atomic force microscopy
Citation
Tomankova Katerina, Kolarova Hana, Vachutka Jaromir, Zapletalova Jana, Hanakova Adela, Kaplova Eva. Study of photodynamic, sonodynamic and antioxidative influence on HeLa cell line. Indian Journal of Biochemistry & Biophysics. 2014 Feb; 51(1): 19-28.