nspA Gene as a specific genetic marker for detection of Neisseria meningitidis causing bacterial meningitis.
Loading...
Date
2014-06
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Bacterial meningitis caused by Neisseria meningitidis which causes human brain meninges damage, is generally diagnosed from patient cerebrospinal fluid through microscopy, immunological assays, biochemical test, PCR, microarray and biosensors. However, these methods are expensive, time-consuming or non-confirmatory due to certain limitations. A quick PCR based method was developed for detection of bacterial meningitis caused by N. meningitidis using specific primers based on amplification of virulence nspA (Neisseria surface protein A) gene partial sequence (202 bp). The nspA gene amplicon could be used as a genetic marker for minimum detection of 10 ng genomic DNA (G-DNA) of N. meningitidis with high sensitivity only in 80 min, which is least time reported for the confirmation of the disease. However, the lower detection limit was found as low as 1.0 ng G-DNA, but with less sensitivity. The cross-reactivity of the genetic marker was also studied with other possible pathogens. A comparison with the presently available detection methods and our method was also done using patient samples.
Description
Keywords
Genetic marker, Meningitis, Neisseria meningitidis, nspA gene
Citation
Bhatt Neha, Khan Nazneen, Dash Sandip K, Khare Shashi, Kumar Ashok. nspA Gene as a specific genetic marker for detection of Neisseria meningitidis causing bacterial meningitis. Indian Journal of Biochemistry & Biophysics. 2014 Jun; 51(3): 211-214.