Assessment of phagosomes infected with Mycobacterium tuberculosis as a vaccine candidate against tuberculosis.
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Date
2014-11
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Abstract
The present study describes a novel and simple vaccination strategy that involve culturing of M. tuberculosis in the macrophage cells. Isolation of phagosome from macrophage (cell line J774) infected with M. tuberculosis (H37) and M. bovis (BCG) at early and late phase of infection was done ensuing the identification and characterization of these phagosome. In vitro study of apoptosis induced by phagosome infected with (H37) and (BCG) was performed. The vaccine candidate with H37 MOI- 1:10 at 3 h, MOI- 1:20 at 1, 1.5, 2.5 and 3 h and BCG MOI- 1:20 at 3.5 h showed percentage apoptosis as 38.64, 39.93, 34.66, 22.56,34.59 and 37.81% respectively. The results designates that macrophages provide cellular niche during infection and illustrate considerable immunogenic property. Novel antigens expressed or secreted by H37 in infected macrophages can provide evidence to be a successful vaccine candidate as it endures enhanced immune response than BCG.
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Keywords
Apoptosis, BCG, Cell line, Mycobacterium tuberculosis, Vaccine
Citation
Sharma Anjana, Parihar Pankaj, Sharma Juhi. Assessment of phagosomes infected with Mycobacterium tuberculosis as a vaccine candidate against tuberculosis. Indian Journal of Experimental Biology. 2014 Nov; 52(11): 1090-1097.