Assessment of phagosomes infected with Mycobacterium tuberculosis as a vaccine candidate against tuberculosis.

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dc.contributor.authorSharma, Anjana
dc.contributor.authorParihar, Pankaj
dc.contributor.authorSharma, Juhi
dc.date.accessioned2014-12-12T11:00:55Z
dc.date.available2014-12-12T11:00:55Z
dc.date.issued2014-11
dc.description.abstractThe present study describes a novel and simple vaccination strategy that involve culturing of M. tuberculosis in the macrophage cells. Isolation of phagosome from macrophage (cell line J774) infected with M. tuberculosis (H37) and M. bovis (BCG) at early and late phase of infection was done ensuing the identification and characterization of these phagosome. In vitro study of apoptosis induced by phagosome infected with (H37) and (BCG) was performed. The vaccine candidate with H37 MOI- 1:10 at 3 h, MOI- 1:20 at 1, 1.5, 2.5 and 3 h and BCG MOI- 1:20 at 3.5 h showed percentage apoptosis as 38.64, 39.93, 34.66, 22.56,34.59 and 37.81% respectively. The results designates that macrophages provide cellular niche during infection and illustrate considerable immunogenic property. Novel antigens expressed or secreted by H37 in infected macrophages can provide evidence to be a successful vaccine candidate as it endures enhanced immune response than BCG.en_US
dc.identifier.citationSharma Anjana, Parihar Pankaj, Sharma Juhi. Assessment of phagosomes infected with Mycobacterium tuberculosis as a vaccine candidate against tuberculosis. Indian Journal of Experimental Biology. 2014 Nov; 52(11): 1090-1097.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/153795
dc.language.isoenen_US
dc.source.urihttps://nopr.niscair.res.in/handle/123456789/29750en_US
dc.subjectApoptosisen_US
dc.subjectBCGen_US
dc.subjectCell lineen_US
dc.subjectMycobacterium tuberculosisen_US
dc.subjectVaccineen_US
dc.subject.meshAnimals
dc.subject.meshAntigens, Bacterial --immunology
dc.subject.meshApoptosis
dc.subject.meshCell Line, Tumor
dc.subject.meshCulture Media
dc.subject.meshDNA Fragmentation
dc.subject.meshLymphoma, Non-Hodgkin --pathology
dc.subject.meshMacrophages --microbiology
dc.subject.meshMice
dc.subject.meshMice, Inbred BALB C
dc.subject.meshMycobacterium bovis --growth & development
dc.subject.meshMycobacterium bovis --immunology
dc.subject.meshMycobacterium tuberculosis --growth & development
dc.subject.meshMycobacterium tuberculosis --immunology
dc.subject.meshPhagosomes --immunology
dc.subject.meshPhagosomes --microbiology
dc.subject.meshTuberculosis Vaccines --immunology
dc.subject.meshTuberculosis Vaccines --isolation & purification
dc.titleAssessment of phagosomes infected with Mycobacterium tuberculosis as a vaccine candidate against tuberculosis.en_US
dc.typeArticleen_US
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