Expression and role of miR-34a in bladder cancer.
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Date
2013-04
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Abstract
To investigate the biological function of microRNA-34a (miR-34a) in bladder cancer, the expression of miR-34a was determined using quantitative real-time polymerase chain (qRT-PCR) reaction in 42 cases of bladder cancer. The relationship between the expression of miR-34a and development of bladder cancer was also studied. The mature mimics of miR-34a were chemically synthesized and transiently transfected into human bladder cancer T24 cells. The effects of miR-34a on apoptosis, cell cycle and proliferation in T24 cells were evaluated by flow cytometry and MTT, respectively. The results showed that the low expression rate of miR-34a was correlated with the malignancy and tumor size of bladder cancer. The up-regulation of miR-34a in T24 cells contributed to cell growth and cell cycle arrest, but not caspase-3 pathway. These findings suggest that the relative low expression of miRNA-34a might be involved in the tumorigenesis of bladder cancer.
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Keywords
MicroRNA-34a, Bladder cancer, Pathological variables, Proliferation, Apoptosis
Citation
Wang Wei, Li Tan, Han Guang, Li Ying, Shi Li-hua, Li Hui. Expression and role of miR-34a in bladder cancer. Indian Journal of Biochemistry & Biophysics. 2013 Apr; 50(2): 87-92.