Cloning and characterization of a gene encoding novel zinc finger protein transcription factor induced under water deficit stress from rice (Oryza sativa) cv. N-22.
Loading...
Date
2012-02
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
A gene OsZnI encoding Cys3/His1-type zinc finger protein was isolated from the water stress-induced cDNA library of rice (Oryza sativa) cv. N-22, an early maturing, deep-rooted, drought-tolerant genotype adapted to upland conditions. The in-silico analysis revealed an insert of 800 bp with an ORF of 663 nucleotides, encoding 221 amino acids. OsZnI had three distinct features — nuclear localization signal (NLS) present in Arg152-Arg168, Zn finger domain between 185-193 amino acids and 12 amino acids conserved domain in 71-82 amino acids homologous to LEA motif, and belonged to C-type family of Zn finger protein. OsZnI showed induced expression under water deficit stress.
Description
Keywords
Drought Stress, Transcription Factor, Zinc Finger Protein, Water Deficit Stress, Rice, Oryza Sativa, N-22
Citation
Soren Khela Ram, Ali Kishwar, Tyagi Aruna. Cloning and characterization of a gene encoding novel zinc finger protein transcription factor induced under water deficit stress from rice (Oryza sativa) cv. N-22. Indian Journal of Biochemistry & Biophysics. 2012 Feb; 49(1): 36-41.