Purification and characterization of bacteriocin produced by strain of Lactobacillus brevis MTCC 7539.
Loading...
Date
2009-08
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Bacteriocin, an antimicrobial agent having potential for food biopreservation was purified from Lactobacillus brevis (a safe food-grade bacteria isolated from Vari Kandal, a traditional fermented food of Himachal Pradesh by adopting a novel repeated washing method. Its purity was confirmed by SDS-PAGE and Native-PAGE. The relative molecular mass of bacteriocin was 93.74 kD, while specific activity and recovery were 35.52 folds and 17.13%, respectively. It showed high thermal stability and was active over wide range of pH and exhibited sensitivity to trypsin.
Description
Keywords
Bacteriocin, Lactibacillus brevis
Citation
Gautam Neha, Sharma Nivedita. Purification and characterization of bacteriocin produced by strain of Lactobacillus brevis MTCC 7539. Indian Journal of Biochemistry & Biophysics. 2009 Aug; 46(4): 337-341.