Journal of Medical Technology and Physical Therapy
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Item Item Detection of Burkholria pseudomallei DNA in whole blood samples of bacterial septicemia patients using conventional PCR and real-time PCR(Khon Kaen University, 2010-04-11) Wisansanee Thaewpia; Medical Technology Department, Khon Kaen; Chonthida Supaprom; Centre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University; Wattanachai Susaengrat; Medicine Department, Khon Kaen Hospital; Chanvit Leelayuwat; Centre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University; Ganjana Lertmemongkolchai; Centre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen UniversityBurkholderia pseudomallei is a bacterium that causes a disease known as melioidosis. Infections of B. pseudomallei appear in several organs including acute septicemia which showed high mortality rate. The rapid and high efficient diagnostic test may reduce the mortality rate of melioidosis patients. We performed conventional PCR using LPS primers and real-time PCR using 16S rDNA primers for detection of B. pseudomallei in clinical blood specimens. Bacterial culture was used as a gold standard. Blood specimens from 32 suspected bacterial septicemia patients were obtained from admitted patients in Khon Kaen Hospital and other hospitals in the region. These consist of the patients with 19 B. pseudomallei and 13 other bacterial infections including 1 Burkholderia cepacia, 1 Escherichia coli, 4 Klebsiella pneumoniae, 1 Enterobacter species, 3 Staphylococcus aureus, 1 Streptococcus pneumoniae and 2 group A Streptococcus. The sensitivity, specificity, positive and negative predictive values were determined. Conventional PCR showed low sensitivity of 37 % (95% CI:15-59) and high specificity of 92 % (95% CI:78-100) with 88 % (95% CI: 65-100) and 50 % (95% CI:30-70) of positive predictive value (PPV) and negative predictive value (NPV), respectively. In contrast, a real-time PCR and using of combination test showed higher sensitivity (63 %, 95% CI: 41-85) and lower specificity (69 %, 95% CI: 44-94) with 75 % (95% CI:54-96) and 56 % (95% CI:32-81) of PPV and NPV, respectively. In 5 melioidosis patients who have died, both PCR methods showed rising of sensitivity [80 % (95 % CI: 45-100) and 100 % (95% CI: 100-100)], respectively. The lower detection limit of B. pseudomallei by conventional PCR was 103 cfu/ml. The conventional PCR and real-time PCR could detect B. pseudomallei DNA of 10 pg and 50 fg per PCR reaction, respectively. The higher sensitivity of real-time PCR may be useful for early screening test, whereas the conventional PCR with higher specificity and PPV may be used as a confirmatory test for diagnosis of B. pseudomallei. Therefore, the two assays may be used in combination as rapid molecular diagnostic for melioidosis which might lead to a reduction in the mortality rate of melioidosis patients.Item Health-promoting behaviors of physical therapy students in Khon Kaen University(Khon Kaen University, 2009-12-24) Thanakorn Thanawat; Nomjit Nualnetr; Wichai EungpinichpongHealth promotion is the responsibility of each person to achieve good health. Health-promoting behaviorsshould be practiced regularly by every one, especially physical therapists who are health promotion providers.During the educational years of physical therapy training, greater emphasis should be placed on the important of anappropriate health-promoting behavior habit to the students. This study aimed to evaluate health-promoting behaviorsof physical therapy students in Khon Kaen University and compare the behaviors among students in each educationalyear. Participants were 144 undergraduate physical therapy students (mean age 20.2\± 1.3 years) who werestudying at the Faculty of Associated Medical Sciences, Khon Kaen University in 2008. Their health-promotingbehaviors were assessed by a self-completed questionnaire namely the Thai health promoting lifestyle profileII (HPLP-II). The questionnaire contains 52 items that evaluate behaviors regarding health responsibility, physicalactivity, nutritional habits, spiritual growth, interpersonal relations, and stress management. The results revealed thatthe participantsํ overall health-promoting behaviors were at a fair level. The 6 subscales of the behaviors rangedfrom good to very poor, with the highest and lowest levels in the interpersonal relations and physical activity,respectively. Significant differences in health-promoting behaviors among students in the 4 educational years werefound in the health responsibility and physical activity (P \< 0.05). These findings could be used as database for thecurriculum planners and faculty administrators to set up a strategy of health promotion, structure healthier environments,and develop health education programs for encouraging physical therapy students to adopt healthy lifestyles.Item J Med Tech Phy Ther on the web Health Science Journals in Thailand(Khon Kaen University, 2010-04-11) Supan FucharoenAbstract not availableItem Screening of anticancer activity from Streptomyces spp.(Khon Kaen University, 2010-04-11) Wipada Ngearnthaworn; Division of Medical Technology, School of Allied Health Sciences and Public Health, Walailak University, Nakorn Si Thammarat; Surapong Klybud; Division of Medical Technology, School of Allied Health Sciences and Public Health, Walailak University, Nakorn Si Thammarat; Sulaiman Awae; Division of Medical Technology, School of Allied Health Sciences and Public Health, Walailak University, Nakorn Si Thammarat; Phuwadol Bangrak; Division of Biology, School of Science, Walailak University; Warangkana Chunglok; Utilization of Natural Product Unit, Walailak University; Monthon Lertcanawanichakul; Utilization of Natural Product Unit, Walailak UniversityThe study was aimed to evaluate anticancer activity from Streptomyces spp. Human mouth carcinoma cell line (CLS-354 cell) was used as a cell model for cytotoxicity testing. Culture broth and whole cell lysates obtained from three isolates of Streptomyces spp. (CO4, WU6 and WU10) were evaluated for anticancer activity in comparison with 5-fluorouracil (5-FU), known as anticancer drug. Anticancer activity was markedly observed from lyophilized culture broth obtained from CO4 \> WU6 \> WU10. The half maximal inhibitory concentration (IC50) obtained from CO4, WU6, and WU10 was 5 mg protein/ml (5.88 mg/ml), 31 mg protein/ml (34.83 mg/ml), and 23 mg protein/ml (25.62 mg/ml), respectively. Whole cell lysates obtained from CO4 revealed IC50 of 78 mg protein/ml. The lysates from WU6 or WU10 revealed IC50 of more than 625 mg protein/ml. This was suggested that lyophilized culture broth obtained from Streptomyces spp. was more potent anticancer activity than did whole cell lysates. Anticancer drug, 5-FU was more efficient than lyophilized culture broth obtained from Streptomyces spp with IC50 of 0.75 mg/ml. This may depend on the purity of bioactive compounds produced from Streptomyces spp. Thus, Streptomyces spp. may provide promising sources of anticancer bioactive agents which need to be further evaluated.Item Identification of Haemophilus spp. from clinical specimens by biochemical tests compared with PCR method(Khon Kaen University, 2010-04-11) Pairshompoo Saetung; Graduate School, Khon Kaen University; Aroonlug Lulitanond; Centre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University; Chotechana Wilailuckana; Centre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University; Aroonwadee Chanawong; Centre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University; Pipat Sribenjalux; Centre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University; Unchalee Janewatana; Department of Pathology, Faculty of Medicine Ramathibodi Hospital, Mahidol UniversityHaemophilus influenzae is a part of normal upper respiratory flora of human, which can cause a wide variety of infections. Other members of genus Haemophilus rarely cause human infection but are frequently isolated from clinical specimens, such as sputum. The pathogenicity between H. influenzae and other Haemophilus species is different therefore a reliable method for identification of H. influenzae is essential. The aim of this study was to compare the identification methods for Haemophilus by four phenotypic tests with that by a PCR-based method. A total of 101 Haemophilus isolates were identified by biochemical tests and the XV requirement test by using XV paper strip technique, porphyrin test and Staphylococcus streak technique. The PCR-based method was performed using specific primers for 16SrDNA, p6 genes of H. influenzae and sodA gene of H. parainfluenzae. Using the XV paper strip technique, porphyrin test and biochemical tests, 88 and 13 isolates were identified as H. influenzae and H. parainfluenzae respectively, whereas 54 H. influenzae and 47 H. parainfluenzae were identified by using Staphylococcus streak technique (66.4 % agreement with that of the three tests). The PCR-based method revealed that 83 H. influenzae and 12 H. parainfluenzae were identified, whereas 6 isolates could not be categorized into both species. This study showed that identification of Haemophilus by the XV paper strip technique, porphyrin test and biochemical test gave 93.1 % agreement with that of the PCR method, whereas the Staphylococcus streak technique gave only 71.3 % agreement.Item Item Differential expression of gene profiling in therapeutic response and non-response cervical carcinoma(Khon Kaen University, 2010-04-11) Montira Sakunjia; Master of Science Program in Medical Sciences, Khon Kaen University; Patcharee Jearanaikoon; Centre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University; Pisamai Yuenyao; Department of Obstetrics and Gynaecology, Faculty of Medicine, Khon Kaen University; Inthira Tussakhon; Temduang Limpaiboon; Centre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen UniversityCervical carcinoma is one of the most common malignancies in women worldwide. Persistent infection with high risk human papillomavirus is the most significant factor for carcinogenesis of cervical cancer. Therapeutic modalities of cervical carcinoma are not efficiently controlling the progression of cancer in one third of patients. Therefore, understanding of molecular mechanisms in cancer progression is important to prevent disease progression and improve treatment efficacy leading to the reduction of incidence and mortality rate. We investigated genes differentially expressed in therapeutic response and non–response cervical carcinoma stage IIIB using the human whole genome microarrays. The differential expression of genes in each group was constructed for the network by IPA software. Differentially expressed genes in non–responses compared with responses were identified, which were 167 up-regulated and 146 down-regulated genes. The up-regulated genes have biological functions involved in signal transduction (CXCL10, ERBB2, TRAF5, FPRL1, IL1B, FGFBP1 and SCTR), cell proliferation (REG1A, AREG, TGFBI, ERBB2, PIM2, EIF5A and ISG20), proteolysis (PLAU, CRADD, C1R, KLK14, TMEM27, ICEBERG, BF and ADAMTS8), and cell migration (ERBB2, IL8, IL1B, and FPRL1). The down-regulated genes have biological functions involved in transcription (GLIS1, ZNF337, SOX4, PITX1, LASS4, GRHL3, CRABP2, SUHW3, TLE2 and KLF8), cell adhesion (PCDHB6, TRO, MAGI1, PVRL4, SLURP1 and MUC4), and signal transduction (CD38, CXCL14, HTR2B, CEACAM6, DPYSL3, NCR1, CRABP2, CLIC3, TLE2 and PLXNA2). Our study indicates that multiple genes play distinct roles in response or non-response to therapy in cervical carcinoma.Item Effects of heparin on thalassemia genes analysis by PCR(Khon Kaen University, 2010-04-11) Kitsada Singha; Faculty of Associated Medical Sciences, Khon Kaen University; Sirintra Chaitongyot; Faculty of Associated Medical Sciences, Khon Kaen University; Goonnapa Fucharoen; Centre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University; Supan Fucharoen; Centre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen UniversityEDTA-anticoagulated blood is usually recommended for use in routine analysis of thalassemia genes. However, collection of blood from the fetus or newborns is usually done using heparin because of its more potent anti-coagulant activity. However, since heparin can inhibit Taq polymerase enzyme used in PCR, the use of heparinized blood for PCR analysis may be affected. ACD, another blood anti-coagulant with a preservative property may be used as an alternative anti-coagulant. In this study, the effects of EDTA, heparin and ACD anti-coagulants on PCR analysis of a – thalassemia 1 (SEA deletion) and bE – globin gene were compared. a – thalassemia 1 was detected using gap – PCR and bE – globin gene was identified by allele specific PCR assay. It was found that both EDTA and ACD anti-coagulants had no effect on the efficiency of PCR analysis of the two thalassemia genes. However, substantial reduction in the efficiency of PCR analysis was observed with the use of heparin as an anti-coagulant at a concentration over 25 IU/ml. Amplification efficiency was improved when heparinized blood DNA was diluted prior to PCR analysis. The result from this study should prove useful for development of a guideline of blood collection for routine PCR analysis of halassemia.Item Item Item Recognition of major methicillin-resistant Staphylococcus aureus (MRSA) clones by molecular surveillance in Srinagarind Hospital, Khon Kaen(Khon Kaen University, 2010-04-11) Boualay Norchaleun; Graduate School, Khon Kaen University; Chotchana Wilailuckana; Centre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University; Nuttanan Hongsrichan; Centre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University; Wanlop Kaewkes; Center for Emerging Diseases, Faculty of Medicine, Khon Kaen University; Chokchai Wilachai; Clinical Microbilogy Laboratory, Srinagarind Hospital, Khon Kaen University; Aroonlug Lulitanond; Centre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University; Aroonwadee Chanawong; Centre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University; Prakai Pitak; Infection control unit, Srinagarind Hospital, Khon Kaen University; Janpen Bourpoern; Infection control unit, Srinagarind Hospital, Khon Kaen University; Saisamorn Poldongnauk; Infection control unit, Srinagarind Hospital, Khon Kaen UniversityMethicillin-resistant Staphylococcus aureus, a well-known nosocomial pathogen in tertiary healthcare facilities, can cause severe life-threatening symptoms. Nowadays, prevention and control of outbreaks related to hospital-acquired infections need molecular information to distinguish and definitely define a real etiology. For the last decade, molecular techniques have been developed and applied to an epidemiological study of infectious diseases. Among them, polymerase chain reaction-based typing techniques are most feasible to be used as molecular tools in clinical microbiology laboratory in Thailand. In this study, PCR-based typing methods, including SCCmec typing, variable numbers of tandem repeats typing of hypervariable region downstream of mecA (HVR) locus and spa gene, were applied in order to determine genetic background, and major endemic clones in Srinagarind Hospital, Khon Kaen. A total of 247 MRSA isolated from 124 patients of Srinagarind Hospital during July 2007 through December 2008 were characterized by the PCR-based typing methods described above. Five SCCmec types were identified as type-III (60.7%), type-IIIA (30.8%), type-II SCCmec (6%), type-III DCS (1.7%), and type-I variant with class C mec complex (0.9%), respectively. HVR and spa typing differentiated MRSA into 5 and 10 groups, respectively. Combination of all genetic markers could identify two major clones, III-15-7 (43.6%), and IIIA-7-7 (22.2%). Medical wards and medical intensive care unit were considered as endemic areas of these two clones. Information in this study may be applied to infection control measure and lead to development of suitable PCR-typing techniques for MRSA in clinical laboratory.Item Proficiency testing in laboratory diagnosis of common thalassemia and hemoglobinopathies in Thailand(Khon Kaen University, 2009-12-24) Goonnapa Fucharoen; Kanokwan Sanchaisuriya; Duangrudee Changtrakun; Nattaya Sae-ung; Yossombat Changtrakun; Supan FucharoenTo improve the efficiency of laboratory diagnosis of common thalassemia and hemoglobinopathies in Thailand,the proficiency testing program was set up at the Centre for Research and Development of Medical DiagnosticLaboratories (CMDL), Khon Kaen University. The KKU-Hb controls were sent to laboratory members togetherwith essential hematological parameters. Each time, two control samples designated as husband and wife were sent.Upon receiving, all laboratory members analyzed control samples in their routine practices and interpreted the resultusing hematological data received and result of Hb analyses in their laboratories. The result of laboratory investigationsand the interpretations as well as the risks of having fetuses with 3 severe thalassemia diseases including homozygousα- thalassemia 1, homozygous β-thalassemia and β-thalassemia / Hb E disease were applied into the form providedand sent back to CMDL. Three cycles were investigated with 21, 23 and 66 participant laboratories, respectively.All control samples were received within appropriate times and conditions. It was found that more than 90 % ofparticipant laboratories could report acceptable levels of Hb A2 and Hb F and give accurate interpretation. Memberswere analyzed and grouped into 4 different quality groups;Excellent, Good, Fair and Need improvement.The proportions of members in the Excellent, Good, Fair and Need improvement groups were respectively found tobe (81.0, 9.5, 4.75 \& 4.75 %) in the first cycle and (69.6, 0, 21.7 \& 8.7 %) in the second cycle and (56.0, 18.2,24.3 \& 1.5 %) in the third cycle. It was found that the values of Hb A2 and Hb F were reported quite accuratelyfrom each laboratory member. However, when samples with complicated data were supplied, the increased inmis-interpretation and evaluation of relative risks were observed. This result indicates the requirement of furtherimprovement in the laboratory interpretation and knowledge related to laboratory diagnosis of thalassemiaand hemoglobinopathies of the participants. The evaluation system developed should prove useful in both developmentof external quality control program in laboratory diagnosis and further facilitate the prevention and control programof thalassemia and hemoglobinopathies in Thailand.Item Helicobacter pylori antibody and antinuclear antibody in chronic urticaria patients(Khon Kaen University, 2010-01-14) Somchai Chanjanakijskul; Daraka Phainupong; Paisal Roongphibulsopit; Malai Areromrat; Rungkul Seangngam; Thongbai Himman-ngnan; Patcharin Janjumratsang; Rungsunn TungtrongchitrHelicobacter pylori antibody and antinuclear antibody (ANA) were investigated in 138 cases of chronic urticaria patients and 96 normal controls. No statistically significant difference of ANA was shown when comparing between the two groups. The prevalence of positive Helicobacter pylori antibody in chronic urticaria was found to be 45.6 % and 21.9 % in normal subjects (p ≤ 0.001). Positive Helicobacter pylori antibody of 37.3 %, 47.6 % and 59.1 % in chronic urticaria patients were detected in the age range of 15-30 years, 31-45 years and 46-60 years respectively. Helicobacter pylori antibody were presented in 14.3 %, 17.5 % and 35.7 % of normal subjects the age range of 15-30 years, 31-45 years and 46-60 years respectively (p = 0.032, 0.002, 0.100). The prevalence of positive Helicobacter pylori antibody in chronic urticaria patients were found to be 2.61, 2.72 and 1.66 times in the age ranges of 15-30 years, 31-45 years and 46-60 years respectively when comparing with positive result in normal subjects.Item α/β Globin mRNA of β-thalassemia genes with premature termination(Khon Kaen University, 2010-01-20) Ratchanok Ngouprommin; Nattaya Sae-ung; Supan Fucharoen; Goonnapa Fucharoen; Kanokwan SanchaisuriyaMutation of β-globin gene causing translation-premature termination results in significant decrease of the mRNA abundance. This phenomenon arises from the degradation of mutant mRNA by nonsense codon-mediated mRNA decay (NMD). In this study, α/β-globin mRNA ratio was determined by semi-quantitative RT-PCR in β-thalassemia carriers with the β17, β41/42, β71/72 and β27 mutations and in a patient with compound heterozygous β17, β27 mutations. Values were compared with normal individual. The α/β-globin mRNA ratio of normal individual was found to be 0.98 whereas those of heterozygous for β17, β41/42, β71/72 and compound heterozygous β17/β27 were 0.89 1.66 1.60 and 3.09, respectively. Direct DNA sequencing of the cDNA demonstrated mutant mRNA only in the carrier with β17 mutation but not other mutations. This result was in concordance with the α/β-globin mRNA ratio observed. This data indicates that the α/β-globin mRNA ratio is dependent on the type and the location of premature termination mutation and related to some other factors involving NMD mechanism in cells.Item Comparison of modified semi - solid Rappaport - Vassiliadis to routine culture media for isolation of Salmonella from rectal swab specimens at Srinagarind Hospital(Khon Kaen University, 2010-01-14) Lumyai Wonglakorn; Seksit Sungkeeree; Prachaub Chaimanee; Amorn Ooncharern; Supaporn Poorpermpoolsiri; Suthida Kenprom; Kanjana JungrungraungSalmonella is an infectious agent of gastrointestinal disease. Rapid isolation and identification of this organism are important for early and effective treatment. Salmonella, as well as other enteric bacteria, shows similar growth and colonies on MacConkey agar and Salmonella-Shigella agar, which are commonly used for isolation of Salmonella in most clinical laboratories. For better isolation and differentiation of this organism from normal enteric flora, modified semi-solid Rappaport-Vassiliadis agar (MSRV) was compared with the routine culture media used in Clinical Microbiology Unit, Srinagarind Hospital. A total of 1,944 rectal swabs were cultured by both methods. It was found that 159 isolates (8.2%) of Salmonella were recovered in this study, 153 isolates (7.9%) from MSRV and 103 isolates (5.3%) from routine medium. Of these, 97 isolates (5.0%) were positive by both methods, 56 isolates (2.9%) were positive by MSRV only, while 6 isolates (0.3%) were positive by routine media only. This study showed that MSRV gave a better result than those of the routine culture media. Therefore, MSRV should be used as additional medium for efficiency of isolation of Salmonella from rectal swab in clinical microbiology laboratories.Item The effect of modified Thai therapeutic massage on pain reduction and knee function in individuals with primary knee osteoarthritis: a randomized control trial(Khon Kaen University, 2010-01-14) Suwattana Klinsrisuk; Wichai Eungpinichpong; Sermsak Sumanant; Uraiwan ChatchawanSee full text in a pdf formatItem Comparison of ultrasound powers between the ultrasound equipment for cosmetic and for physical therapy(Khon Kaen University, 2010-01-14) Siriphan Eamrungroj; Pismai Malila; Wichai Eungpinichpong; Jiraporn Wanpakre; Pakamart Sriring; Mahakraporn PolyiamSee full text in a pdf formatItem Tapasin expression in cervical carcinoma(Khon Kaen University, 2010-01-20) Patcharee Jearanaikoon; Nichapawee Nattasirikul; Wipawee Kaewpo; Surasak Wanram; Chanvit Leelayuwat; Anucha Paupairoj; Temduang LimpaiboonCervical cancer is the most common cancer among women worldwide. Even though HPV infection is known as the major risk factor of cervical carcinogenesis, immune evasion of infected cells or tumor cells is also well established as the major contributing factors. Down regulation of MHC class I as well as the presence of non-surface presenting MHC class I molecules have been previously reported. Our aim of this study is to investigate the expression level of tapasin protein which is one of the MHC class I antigen processing proteins in various stages of cancerous tissues. Seventy nine dysplasia and 49 cases of cervical cancer and 49 normal cervix from myoma patients as control were included in this study. The association between the expression level and disease progression was analyzed using χ2 for trend. The reduction of tapasin expression is significantly associated with respect to the progression of staging in both dysplasia and cervical cancer group (p=0.001; χ2 for trend). In conclusion, our study illustrates that the reduction in tapasin expression might exert as one of the mechanism leading to the impairment of antigen presenting of MHC class I in cancerous tissues. Therefore, tapasin staining might be useful as an early prognostic marker in dysplasia. More antigen processing proteins should be also further investigated.Item Abstracts, The 2007 Annual meeting of the Faculty of Associated Medical Sciences, 177 Khon Kaen University(Khon Kaen University, 2010-01-20) . .Abstract not available