Wongstitwilairoong, BoonchaiSrijan, ApichaiPiyaphong, SongmuangKhungvalert, VitayaChivaratanond, OrapanBodhidatta, LadapornSmith, B LMason, C J2009-05-272009-05-272005-05-30Wongstitwilairoong B, Srijan A, Piyaphong S, Khungvalert V, Chivaratanond O, Bodhidatta L, Smith BL, Mason CJ. Significantly increased recovery of intestinal parasites on routine stool specimen evaluation. The Southeast Asian Journal of Tropical Medicine and Public Health. 2005 May; 36(3): 641-3http://imsear.searo.who.int/handle/123456789/33803The Southeast Asian Journal of Tropical Medicine and Public Health.Three hundred thirty-six stool samples from October 2001 through October 2002 were analyzed for the presence of intestinal parasites. Fifty-six of these (16.7%) were positive for a total of 66 parasites; 65/66 (98.5%) were detected by iodine and dimethyl sulfoxide-modified acid-fast (DMSO-mAFB) stained smears of fresh and formalin-ethylacetate sedimentation concentrated samples. Saline, iodine, and DMSO-mAFB stained smears of fresh stool samples alone detected significantly fewer parasites, finding only 50/66 (75.8%) (p < 0.05). Stool samples analyzed by trichrome stained specimens preserved in Zinc sulfate polyvinyl alcohol (Zinc PVA) detected only 41/ 66 (62.2%) of the parasites. In our study population, it was necessary to perform the National Committee for Clinical Laboratory Standard (NCCLS) recommended to accurately detect intestinal parasites. The concentration technique is simple and significantly increased the detection of intestinal parasites.engAzo Compounds --diagnostic useChild, PreschoolColoring Agents --diagnostic useDiagnostic Tests, Routine --methodsDimethyl Sulfoxide --diagnostic useEosine Yellowish-(YS) --diagnostic useFeces --parasitologyHumansInfantIntestines --parasitologyIodine --diagnostic useMethyl Green --diagnostic useSolvents --diagnostic useSpecimen Handling --methodsThailandTrace Elements --diagnostic useEvaluation Studies