Peter, CDShashidara, RJain, VHaragannavar, VCSamuel, PNayak, SR.2023-08-102023-08-102023-03Peter CD, Shashidara R, Jain V, Haragannavar VC, Samuel P, Nayak SR.. Senescence in oral lichen planus as assessed by the immunohistochemical evaluation of senescence marker protein-30 (Regucalcin). Indian Journal of Pathology & Microbiology. 2023 Mar; 66(1): 9-130377-49290974-5130http://imsear.searo.who.int/handle/123456789/223396Background: Oral lichen planus is a T-cell-mediated chronic inflammatory disease affecting approximately 1% to 2% of the population, the etiology of which is currently unknown. The objectives of this study were to observe if senescence occurs in oral lichen planus, through the assessment of the immunohistochemical expression of a novel marker for senescence called Senescence marker protein-30 or regucalcin, and compare the expression to that in oral lichenoid reaction and non-specific inflammation. Subjects and Methods: The study material consisted of 30 cases of oral lichen planus, 15 cases of oral lichenoid reaction and 15 cases of non-specific inflammation. The number of positive cells in ten randomly selected high power fields were counted in the epithelium and the connective tissue separately and the mean was determined. Results: Mann–Whitney U test was used to statistically analyze if there was any significant difference in the expression of Senescence marker protein-30 between oral lichen planus, oral lichenoid reaction and non-specific inflammation. Even though a greater expression was seen in the oral lichen planus cases than oral lichenoid reaction, the difference in both the epithelium and connective tissue was not statistically significant. Conclusion: This study shows that in addition to the already known mechanisms like apoptosis and increased cell proliferation rates, the activated T-lymphocytes may also trigger a senescent change in the cells of oral lichen planus. As with the other mechanisms, this is also seen only in a small proportion of the cases.Cellular senescenceoral lichen planusoral lichenoid reactionregucalcinsenescence marker protein- 30Journal ArticleIndiaDepartment of Oral Pathology and Microbiology, Educare Institute of Dental Sciences, College Road, Kiliyamannil Campus, Chattiparamba, Malappuram, Kerala, IndiaDepartment of Oral Pathology and Microbiology, Coorg Institute of Dental Sciences, Kodagu, Karnataka, IndiaDepartment of Public Health Dentistry, KLE Society's Institute of Dental Sciences, Bengaluru, Karnataka, IndiaDepartment of Oral Pathology and Microbiology, M.S Ramaiah University of Applied Sciences, Bangalore, Karnataka, IndiaDepartment of Prosthodontics, Educare Institute of Dental Sciences, Malappuram, Kerala, India