Sridhar Rao, P NBasavarajappa, K GKrishna, G Leela2008-10-152009-05-292008-10-152009-05-292008-10-15Sridhar Rao PN, Basavarajappa KG, Krishna GL. Detection of extended spectrum beta-lactamase from clinical isolates in Davangere. Indian Journal of Pathology & Microbiology. 2008 Oct-Dec; 51(4): 497-9http://imsear.searo.who.int/handle/123456789/73356Extended spectrum beta-lactamase enzymes (ESBLs) are enzymes that have the ability to hydrolyze oxyiminocephalosporins and infections by isolates producing them are often difficult to treat. A study to detect the presence of these enzymes in isolates was conducted by our hospital. A total of 207 non repetitive isolates were screened for resistance to any of five screening agents. Those with suspicious profiles were checked for ESBL production by double-disk approximation or a synergy test. The isolates were also subjected to a phenotypic confirmation test as recommended by CLSI (formerly NCCLS). Various cephalosporins-beta-lactamase inhibitor combinations were also tested. Of the 204 (98.5%) screen-positive isolates, only 126 (61.7%) were identified as ESBL producers. Of these, 26.1% of the isolates were positive by using the double-disk synergy test (DDST) method alone, 13.4% were positive using the method recommended by CLSI, and 60.3% of the isolates were positive by both the DDST and CLSI methods. We also report a high percentage of resistance to cefoxitin (96.8%) indicating changes in porins.engAcademic Medical CentersAnti-Bacterial Agents --pharmacologyCephalosporin ResistanceCephalosporins --pharmacologyEnterobacteriaceae --classificationEnterobacteriaceae Infections --microbiologyFemaleHumansMaleMicrobial Sensitivity Tests --methodsbeta-Lactam Resistancebeta-Lactamases --biosynthesisbeta-Lactams --pharmacologyDetection of extended spectrum beta-lactamase from clinical isolates in Davangere.Journal Article