Ivanov, Ivan G.Vrancheva, Radka Z.Petkova, Nadezhda T.Tumbarski, YulianIvayla, N. DinchevaBadjakov, Ilian K.2020-10-162020-10-162019-02Ivanov Ivan G., Vrancheva Radka Z., Petkova Nadezhda T., Tumbarski Yulian, Ivayla N. Dincheva, Badjakov Ilian K.. Phytochemical compounds of anise hyssop (Agastache foeniculum) and antibacterial, antioxidant, and acetylcholinesterase inhibitory properties of its essential oil. Journal of Applied Pharmaceutical Science. 2019 Feb; 2019 Feb: 072-0782231-3354http://imsear.searo.who.int/handle/123456789/210525Agastache foeniculum (Pursh) Kuntze (anise hyssop) is known as a medicinal and flavoring spice plant. The aim ofthis research was gas chromatography-mass spectrometry (GC-MS) analysis of anise hyssop essential oil and analysisof radical scavenging ability, antimicrobial activity, and acetylcholinesterase inhibitory activity. Phytochemicalcomposition of anise hyssop wastes and the aqueous extract obtained after steam distillation was investigated.Eight compounds were identified in essential oils by GC-MS analysis, as the major ones were phenylpropenoids[estragol—93.45% of total ion current (TIC), eugenol—0.15% of TIC, and methyl isoeugenol—2.48% of TIC]. Inthe anise hyssop wastes extract, four pentacyclic triterpenes were identified (betulin—36.1 mg/g, betulinic acid—2.4mg/g, oleanolic acid—160.0 mg/g, and ursolic acid—6.7 mg/g extract). Rosmarinic acid (50.6 mg/g extract) andflavonoids—myricetin (0.5 mg/g), luteolin (0.9 mg/g), and apigenin (0.6 mg/g) were detected by high-performanceliquid chromatography with diode-array detection analysis of aqueous extract. The anise hyssop essential oil showedstrong radical scavenging ability IC50—6.54 μl/ml. The results obtained from antimicrobial screening revealed thatessential oil possessed inhibitory activity against Staphylococcus aureus ATCC 25923, Curtobacterium flaccumfaciensPM_YT, Listeria monocytogenes, Bacillus subtilis ATCC 6633, Salmonella sp., Escherichia coli ATCC 8739, Proteusvulgaris, Pseudomonas aeruginosa ATCC 9027, Klebsiella pneumoniae, and Candida albicans, while Enterococcusfaecalis remained unaffected.Agastache foeniculumessential oilpentacyclic triterpenesantimicrobial activityantioxidant activityrosmarinic acid.Journal ArticleIndiaDepartment of Organic Chemistry and Inorganic Chemistry, University of Food Technologies, Plovdiv, BulgariaDepartment of Analytical Chemistry and Physical Chemistry, University of Food Technologies, Plovdiv, BulgariaDepartment of Organic Chemistry and Inorganic Chemistry, University of Food Technologies, Plovdiv, Bulgaria.Department of Microbiology, University of Food Technologies, Plovdiv, BulgariaAgroBioInstitute, Agricultural Academy, Sofia, BulgariaAgroBioInstitute, Agricultural Academy, Sofia, Bulgaria