Kumar, B SanthoshSandhyamani, SNazeer, Shaiju SJayasree, R S2015-05-052015-05-052015-02Kumar B Santhosh, Sandhyamani S, Nazeer Shaiju S, Jayasree R S. Rapid and simple method of photobleaching to reduce background autofluorescence in lung tissue sections. Indian Journal of Biochemistry & Biophysics. 2015 Feb; 52 (1): 107-110.http://imsear.searo.who.int/handle/123456789/157962Autofluorescence exhibited by tissues often interferes with immunofluorescence. Using imaging and spectral analysis, we observed remarkable reduction of autofluorescence of formalin fixed paraffin embedded tissues irradiated with light prior to incubation with immunofluorescent dyes. The technique of photobleaching offers significant improvement in the quality and specificity of immunofluorescence. This has the potential for better techniques for disease diagnosis.enAutofluorescenceImmunofluorescencePhotobleachingAnti-nuclear antibodyAntibodies, Antinuclear --diagnostic useFluorescent Antibody Technique --methodsLung --cytologyParaffin Embedded --menthodsPhotobleachingSpectrophotometry, Fluorescence --methodsArticle