Karmakar, SMuhuri, D CDasgupta, S CNagchaudhuri, A KGomes, A2009-05-282009-05-282004-05-06Karmakar S, Muhuri DC, Dasgupta SC, Nagchaudhuri AK, Gomes A. Isolation of a haemorrhagic protein toxin (SA-HT) from the Indian venomous butterfish (Scatophagus argus, Linn) sting extract. Indian Journal of Experimental Biology. 2004 May; 42(5): 452-60http://imsear.searo.who.int/handle/123456789/55694A haemorrhagic protein toxin (SA-HT) was isolated and purified from the spine extract of the Indian venomous butterfish, S. argus Linn, by two step ion exchange chromatography. The toxin was homogeneous in native and SDS-PAGE gel. SDS-molecular weight of the toxin was found to be 18.1 +/- 0.09 kDa. SA-HT produced severe haemorrhage on stomach wall but devoid of cutaneous haemorrhage. UV, EDTA, trypsin, protease, cyproheptadine, indomethacin, acetylsalicylic acid and BW755C treatment significantly antagonized the haemorrhagic activity of SA-HT. The toxin produced dose and time dependent oedema on mice hind paw, which was significantly encountered by cyproheptadine, indomethacin and BW755C. SA-HT increased capillary permeability on guinea pig dorsal flank. On isolated guineapig ileum, rat fundus and uterus, SA-HT produced slow contraction which was completely antagonised by prostaglandin blocker SC19220. On isolated rat duodenum, SA-HT produced slow relaxation. SA-HT significantly increased plasma plasmin, serum MDA level and decreased serum SOD level indicating the possible involvement of cyclooxygenase and lipooxygenase pathway.eng4,5-Dihydro-1-(3-(trifluoromethyl)phenyl)-1H-pyrazol-3-amine --pharmacologyAnimalsAnti-Inflammatory Agents, Non-Steroidal --pharmacologyAspirin --pharmacologyCapillariesChromatography, Ion ExchangeCyproheptadine --pharmacologyDose-Response Relationship, DrugEdema --chemically inducedEdetic Acid --pharmacologyElectrophoresis, Polyacrylamide GelFemaleFish Proteins --chemistryFish Venoms --chemistryGastrointestinal Agents --pharmacologyGuinea PigsIndomethacin --pharmacologyLipoxygenase --metabolismMiceMuscle, Smooth --drug effectsPerciformesPermeabilityRatsSpine --metabolismSuperoxide Dismutase --metabolismTime FactorsTrypsin --pharmacologyUltraviolet RaysUterus --drug effectsJournal Article