Mesquita, Harleson LopesFontes, Livia Beatriz AlmeidaCinsa, Laetitia AlvesAdemar, Alves Da Silva FilhoAkinori, Cardozo NagatoBeatriz, Julião Vieira AarestrupJosé, Otávio do Amaral CorrêaFernando, Monteiro Aarestrup2020-10-162020-10-162019-07Mesquita Harleson Lopes, Fontes Livia Beatriz Almeida, Cinsa Laetitia Alves, Ademar Alves Da Silva Filho, Akinori Cardozo Nagato, Beatriz Julião Vieira Aarestrup, José Otávio do Amaral Corrêa, Fernando Monteiro Aarestrup. β-Caryophyllene causes remyelination and modifies cytokines expression in C57BL/6 mice with experimental autoimmune encephalomyelitis. Journal of Applied Pharmaceutical Science. 2019 Jul; 2019 Jul: 027-0332231-3354http://imsear.searo.who.int/handle/123456789/210400The aim of this study is to evaluate the effect of β-Caryophyllene (BCP) on the production of IL-17, transcription factors(T-bet and GATA-3), and remyellination in C57BL/6 mice induced for experimental autoimmune encephalomyelitis(EAE), the model for studying pathogenesis and new therapies for multiple sclerosis. EAE was induced in threegroups of C57BL/6, with administration of BCP in two groups (25 and 50 mg/kg/day) by gavage, after the 10th dayof induction. At 9 days of treatment, mice were euthanized and CNS was removed for the analysis. The profiles ofIL-17, T-bet, GATA-3, and the possible remyelination properties were investigated in the central nervous system(CNS) by immunohistochemistry and Weigert–Pal–Russel’s method, respectively. BCP group (50 mg/kg/day) showeda reduction of IL-17 in brain, cerebellum, and medulla (p < 0.05) and a decrease of T-bet (p < 0.05) in medullaand cerebellum, while GATA-3 was increased (p < 0.05) in cerebellum. In both BCP-treated groups were observedremyelination and better organization of myelin. In conclusion, BCP possesses markedly in vivo anti-inflammatoryand neuroprotective activities and remyelination properties in EAE-mice.β-Caryophyllenemultiple sclerosisexperimental autoimmune encephalomyelitis.β-Caryophyllene causes remyelination and modifies cytokines expression in C57BL/6 mice with experimental autoimmune encephalomyelitisJournal ArticleIndiaLaboratory of Experimental Imunology and Patology, CBR, Federal University of Juiz de Fora, Juiz de Fora, BrazilLaboratory of Experimental Imunology and Patology, CBR, Federal University of Juiz de Fora, Juiz de Fora, Brazil.Laboratory of Experimental Imunology and Patology, CBR, Federal University of Juiz de Fora, Juiz de Fora, BrazilDepartment of Pharmaceutical Sciences, Faculty of Phamarcy, Federal University of Juiz de Fora, Juiz de Fora, BrazilLaboratory of Experimental Imunology and Patology, CBR, Federal University of Juiz de Fora, Juiz de Fora, Brazil.Laboratory of Experimental Imunology and Patology, CBR, Federal University of Juiz de Fora, Juiz de Fora, Brazil.Department of Pharmaceutical Sciences, Faculty of Phamarcy, Federal University of Juiz de Fora, Juiz de Fora, BrazilLaboratory of Experimental Imunology and Patology, CBR, Federal University of Juiz de Fora, Juiz de Fora, Brazil