Sreejith, PBeyo, R SDivya, LVijayasree, A SManju, MOommen, O V2007-06-272009-05-272007-06-272009-05-272007-06-27Sreejith P, Beyo RS, Divya L, Vijayasree AS, Manju M, Oommen OV. Triiodothyronine and melatonin influence antioxidant defense mechanism in a teleost Anabas testudineus (Bloch): in vitro study. Indian Journal of Biochemistry & Biophysics. 2007 Jun; 44(3): 164-8http://imsear.searo.who.int/handle/123456789/26933The effect of the hormones triiodothyronine (T3) and melatonin on antioxidant defense system was studied in 6-propyl thiouracil (6-PTU)-treated or photoperiod-exposed teleost Anabas testudineus. 6-PTU (2 microg/g) treatment or photoperiod exposure (24 h) increased malondialdehyde (MDA) and conjugated dienes (CD) concentrations, indicating increased lipid peroxidation (LPO) in the experimental conditions. T3 or melatonin (10(-6) M) treatment for 15 min in vitro in PTU-treated fish reversed the activity of superoxide dismutase (SOD), catalase and glutathione content. T3-treated group showed no change in glutathione peroxidase (GPx) activity, whereas melatonin treatment decreased its activity. T3 inhibited glutathione reductase (GR) activity. Photoperiod exposure (physiological pinealotomy) induced a stressful situation in this teleost, as evidenced by LPO products and antioxidant enzyme activities. Melatonin and T3 treatment for 15 min in vitro also reversed the effect of photoperiod on peroxidation products and the SOD and catalase activities. GR activity decreased in photoperiod-exposed group and melatonin and T3 treatment reversed the activities. The antioxidant enzymes responded to the stress situation after 6-PTU treatment and photoperiod exposure by altering their activities. The study suggested an independent effect of T3 and melatonin on antioxidant defence mechanism in different physiological situations in fish.engAnimalsAntioxidants --metabolismFishesFree RadicalsGlutathione Peroxidase --metabolismHypothyroidism --metabolismLightLipid PeroxidationLiver --metabolismMelatonin --metabolismPineal Gland --metabolismPropylthiouracil --chemistryThyroid Gland --metabolismTriiodothyronine --pharmacologyJournal Article