Lavhale, Manish SMishra, S H2009-05-282009-05-282007-04-05Lavhale MS, Mishra SH. Evaluation of free radical scavenging activity of Butea monosperma Lam. Indian Journal of Experimental Biology. 2007 Apr; 45(4): 376-84http://imsear.searo.who.int/handle/123456789/58318In the present study, ethyl acetate, butanol and aqueous fractions derived from total methanol extract of Butea monosperma flowers were evaluated for radical scavenging activities using different in vitro models like reducing power assay, scavenging of 2,2 diphenyl-1-picrylhydrazyl (DPPH) radical, nitric oxide radical, superoxide anion radical, hydroxyl radical and inhibition of erythrocyte hemolysis using 2, 2' azo-bis (amidinopropane) dihydrochloride (AAPH). Methanol extract along with its ethyl acetate and butanol fractions showed potent free radical scavenging activity, whereas aqueous fraction was found to be devoid of any radical scavenging properties. The observed activity could be due to the higher phenolic content in the extracts (16.1, 25.29, and 17.74% w/w in methanol extract, ethyl acetate and butanol fractions respectively). HPTLC fingerprint profile of the ethyl acetate and butanol fractions were developed which would serve as reference standard for quality control of the extracts.eng1-Butanol --chemistryAcetates --chemistryBiphenyl Compounds --chemistryButea --chemistryErythrocytes --drug effectsFlowers --chemistryFree Radical Scavengers --isolation & purificationFree Radicals --chemistryHemolysis --drug effectsHydrazines --chemistryHydroxyl Radical --chemistryMethanol --chemistryNitric Oxide --chemistryOxidation-ReductionPlant Extracts --pharmacologySuperoxides --chemistryWater --chemistryJournal Article