Dhume, R AShamayev, N NZhuravlev, B VSudakov, K VSharma, K N1990-10-012009-05-271990-10-012009-05-271990-10-01Dhume RA, Shamayev NN, Zhuravlev BV, Sudakov KV, Sharma KN. Effects of medial septal stimulation & its blockade with atropine on hippocampal rhythmical slow activity in free-moving rabbits. Indian Journal of Medical Research. 1990 Oct; 92(): 353-7http://imsear.searo.who.int/handle/123456789/24177The influence of the medial septal nucleus (MSN) on hippocampal rhythmical slow activity (RSA) was investigated in hunger motivated free-moving rabbits. Single unit activity of 29 CA3 dorsal hippocampal neurones was studied under two paradigms viz., electrical stimulation (3v/1msec/5Hz) and chemical blockade of atropine-sensitive medial septal cells (dose of 5 micrograms in 5 microliters). The analysis of interspike interval sample histograms recorded with Apple II, along with single unit neuronal spikes recorded on polygraph showed bimodal pattern with two peaks; one at bin 10-20 (high frequency) and second at bin 100-300 (theta frequency). On stimulation of MSN, there was total suppression of bin 100-300 with peaks at bin 1-20 and at bin greater than 1000 (very low frequency). Blockade of cholinergic receptors at MSN showed reduction of bin 100-300 and peak at bin 700- greater than 1000; this was followed by gradual decrease of spike's amplitude and thereafter its total abolition. The results indicate that the septal region is not a pacemaker per se in the genesis of hippocampal RSA (theta rhythm), but serves more in the nature of a relay station for a number of inputs and that its influence on the hippocampal electrical activity depends on the integrity of cholinergic inputs, be it from hippocampus proper or from mid-brain reticular formation.engAnimalsAtropine --pharmacologyElectric StimulationHippocampus --cytologyHunger --physiologyMotor ActivityNeurons --physiologyPeriodicityRabbitsSeptal Nuclei --physiologyJournal Article