Harishankar, AnushaChandy, MammenBhattacharya, Sanjay2016-08-082016-08-082015-10Harishankar Anusha, Chandy Mammen, Bhattacharya Sanjay. How to develop an in-house real-time quantitative cytomegalovirus polymerase chain reaction: Insights from a cancer centre in Eastern India. Indian Journal of Medical Microbiology. 2015 Oct-Dec; 33(4): 482-490.http://imsear.searo.who.int/handle/123456789/176501Development of a reliable, cost-effective cytomegalovirus quantitative polymerase chain reaction (QPCR) is a priority for developing countries. Manufactured kits are expensive, and availability can be inconsistent. Development of an in-house QPCR kit that is reliable and quality assured requires significant effort and initial investment. However, the rewards of such an enterprise are manifold and include an in-depth understanding of molecular reactions, and expertise in the development of further low-cost molecular kits. The experience of an oncology centre in Eastern India has been shared. Hopefully, this would provide a brief roadmap for such an initiative. Staff with adequate understanding of molecular processes are essential along with vital infrastructure for molecular research and development.enCytomegalovirusstandardisationtroubleshootingvalidationviral loadArticle