Phyu Phyu WinTin Aye2009-06-102009-06-101989-04-011989-04-01Phyu Phyu Win, Tin Aye. Application of modified enzyme-linked immunosorbent assay (ELISA) for detecting virulent strains of enteroinvassive E.coli and Shigella. Burma Health Sciences Research Journal. 1989; 1(1): 24-28http://imsear.searo.who.int/handle/123456789/125328A virulent isolate of Shigella dysenteriae 1 strain, HW 829 wasd used for the production of specific antibody. Three rabbits (Japanese White strain) were immunized and the serum was obtained. These sera were absorbed with a virulent derivative of the same strain (i.e. HW 829). Tube agglutination titer of immune sera was varied from 1:1,280 to 1:5,120 with living and 1:5,120 to 1:20, 480 with boiled cells of the homologous strain. The absorbed sea were utilized as an antibody in ELISA assay using BIOTIN kit. The investigation of 33 Shigella and 22 enteroinvasive E. coli (EIEC) strains indicated complete agreement between ELISA results and those of the other virulence tests (Sereny test and HEP-2 tissue culture assay).Enzyme-Linked Immunosorbent AssayShigellaEscherichia Coli