Gurumurthy, PremaGayathri, T NBhagavathy, SVenkatesan, P2009-05-282009-05-282004-01-28Gurumurthy P, Gayathri TN, Bhagavathy S, Venkatesan P. Standardization of the method for estimation of ethambutol in pharmaceutical preparations and biological fluid. Indian Journal of Experimental Biology. 2004 Jan; 42(1): 68-73http://imsear.searo.who.int/handle/123456789/60750A simple column chromatographic method for determination of ethambutol (EMB) in pharmaceutical preparations containing EMB in combination with other anti-TB drugs is presented. The method involved extraction of EMB into an organic solvent, followed by basification and column chromatographic separation on Amberlite CG 50 (100-200 mesh) and elution with suitable eluants and estimation at a wavelength of 270 nm. The assay was linear from 25 to 400 microg/ml. The relative standard deviations of intra and inter day assays were lower than 5%. Ethambutol was recovered from human urine quantitatively and stable for a period of at least one week in urine stored at -20 degrees C.engAntitubercular Agents --pharmacokineticsChemistry, Clinical --methodsChromatography --methodsDose-Response Relationship, DrugEthambutol --pharmacokineticsHIV Seropositivity --complicationsHumansIsoniazid --chemistryPharmaceutical PreparationsPyrazinamide --chemistryReference StandardsReproducibility of ResultsResins, Synthetic --chemistrySensitivity and SpecificityTime FactorsJournal Article