Afolabi, A OOluwakanmi, E TSalahdeen, H MOyekunle, A OAlagbonsi, I A2016-03-012016-03-012014-01-01Afolabi A O, Oluwakanmi E T, Salahdeen H M, Oyekunle A O, Alagbonsi I A. Antinociceptive Effect of Ethanolic Extract of Hybanthus enneaspermus Leaf in Rats.British Journal of Medicine and Medical Research. 2014 Jan; 4(1): 322-330.http://imsear.searo.who.int/handle/123456789/174903Aims: To test the hypothesis that Hybanthus enneaspermus leaf has an antinociceptive effect. Methodology: Seventy-two male rats were randomly divided in a blinded fashion into 4 groups each for the tail immersion test (n=12 per group) and formalin test (n=6 per group). Group 1 (control) received 0.6 ml of distilled water. Group 2 received 100 mg/kg of acetaminophen (paracetamol). Group 3 and 4 received 500 mg/kg and 1000 mg/kg of ethanolic extract of Hybanthus enneaspermus leaf (EEHE) respectively. Results: In the formalin test, oral administration of 500 mg/kg and 1000 mg/kg EEHE caused inhibitions of 62.48% and 72% in the early phase and 70.54% and 78.63% in the late phase respectively. The 1000 mg/kg dose significantly reduced the paw licking time when compared to the standard drug (acetaminophen) in the formalin test. The 500 mg/kg and 1000 mg/kg doses significantly increased the tail flick latency in a manner comparable to acetaminophen. Conclusion: This study showed that the leaf has an anti-nociceptive effect.enAcetaminophenanalgesicformalin testHybanthus enneaspermuspaintail flick testArticle