Das, BananiRoy, KalyanTalukdar, Anupam DasDey, AmitabhaNongalleima, KhumukchamDas, SupriyaSharma, PrasantiChoudhury, Manabendra DuttaDeb, Lokesh2023-08-092023-08-092023-01Das Banani, Roy Kalyan, Talukdar Anupam Das, Dey Amitabha, Nongalleima Khumukcham, Das Supriya, Sharma Prasanti, Choudhury Manabendra Dutta, Deb Lokesh. Antifertility activity of Oroxylum indicum (L.) Kurz:In vitro and in vivo study on human sperm and male wistar rats. Indian Journal of Experimental Biology. 2023 Jan; 61(1): 33-410975-10090019-5189http://imsear.searo.who.int/handle/123456789/222592Oroxylum indicum (L.) Kurz, commonly called as Broken bones tree or Indian trumpet flower, belonging to Fam. Bignoniaceae, is traditionally used as a contraceptive by ethnic people of Tripura, North-East India. Here, we investigated the scientific basis for use of O. indicum as male antifertility agent by folklore healers. In vitro spermicidal activity of aqueous (AEOI) and methanolic (MEOI) extracts of O. indicum stem bark were studied on human sperm. The in vivo activity was experimented on male albino rats. The treated animals were allowed to mate and the pups delivered by female rat partners were counted. Phytochemical estimation of test samples was done using HPLC. The AEOI and MEOI treatments significantly decreased human sperm motility and viability. Test extracts have increased the hypo-osmotic swelling of sperm. Both the extracts were significantly declined the weight of reproductive organ. The MEOI treated rats have shown significant decrease in sperm motility and sperm counts. AEOI and MEOI treatment significantly reduced level of testosterone, but sharply raised dihydrotestosterone and prostaglandin in rats. Results testified the traditional claim for use of O. indicum as a male contraceptive agent, where MEOI have shown reversible action on male reproductive system leading to contraception without harming the libido.BaicalinBroken bones treeContraceptiveIndian trumpet flowerKampongOroxylinSperm motilityTestosteroneJournal ArticleIndiaEthnobotany and Medicinal Plant Research laboratory, Department of Life Science and Bioinformatics, Assam University, Silchar-788 011, IndiaDepartment of Pharmacology, Himalayan Pharmacy Institute, Majhitar, Rangpo, Sikkim-737 136, IndiaPharmacology Laboratory, Natural Product Chemistry & Pharmacology Programme, Institute of Bioresources and Sustainable Development (IBSD), Takyelpat, Imphal-795 001, Manipur, IndiaInstitute of Pharmacy, Harish Chandra PG College, Varanasi-221 002, Uttar Pradesh, IndiaInstitute of Bioresources and Sustainable Development (IBSD) - Regional Centre Sikkim, Tadong, Gangtok-795 001, Sikkim, India