Solanki, Khushal SinghQureshi, SalauddinKumar, PawanSahoo, MonalisaM., SaminathanU.K., DeChaudhuri, Pallab2020-10-162020-10-162019-02Solanki Khushal Singh, Qureshi Salauddin, Kumar Pawan, Sahoo Monalisa, M. Saminathan, U.K. De, Chaudhuri Pallab. Patho-Physiological Response of LPS Defective Brucella abortus S19Δper in Experimentally Infected Mice. Journal of Animal Research. 2019 Feb; 9(1): 79-852249-66292277-940Xhttp://imsear.searo.who.int/handle/123456789/210779Brucellosis is an anthropozoonotic infectious disease in which infected animals pose a threat to humans. This disease has considerable social, economic and international trade importance. Despite past and current efforts to eradicate brucellosis, a large number of new human cases are reported annually worldwide. In this study, patho-physiological response to Brucella abortus S19Δper infection in BALB/c mice was assessed in comparison to its parent strain, B. abortus S19 and virulent strain, B. abortus 544. Immunohistochemical analysis confirmed the presence of bacteria in liver and spleen. Comparatively lower serum Aspartate aminotranferase (AST) level and observation of less number of microgranulomas in liver indicated that B. abortus S19 Δper was less infectious and failed to cause active infection unlike S19 and 544 strain. S19Δper, thus could be a safer vaccine candidate as an alternate to the S19 vaccine strain. Hematological studies indicated clinical manifestation of thrombocytopenia in different Brucella infected mice including S19Δper. Therefore, measurement of platelets count and serum AST level may offer as reliable indicators of brucellosis in clinical casesBrucellosisperosamine synthetaselipopolysaccharidesthrombocytopeniaaspartate aminotransferasemicrogranulomaPatho-Physiological Response of LPS Defective Brucella abortus S19Δper in Experimentally Infected MiceJournal ArticleIndiaDivision of Veterinary Biotechnology, ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, INDIADivision of Biological Standardization, ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, INDIADivision of Veterinary Pathology,ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, INDIADivision of Veterinary Pathology,ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, INDIADivision of Veterinary Pathology,ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, INDIADivision of Veterinary Medicine, ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, INDIADivision of Bacteriology and Mycology, ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, INDIA