Mishra, Mahendra NSharma, Ajay2012-07-122012-07-122012-01Mishra Mahendra N, Sharma Ajay. Recombination in human leukocyte antigen region in two Asian Indian families. Indian Journal of Human Genetics. 2012 Jan; 18(1): 109-111.http://imsear.searo.who.int/handle/123456789/139454BACKGROUND: Recombination (crossing over) may generate novel haplotypes that can be beneficial to a population against recently introduced pathogens. It may lead to the generation of new alleles. SETTINGS AND DESIGN: A prospective study at a tertiary care centre. AIM: To report two rare cases of crossing over in HLA region. MATERIALS AND METHODS: Tissue-typing was done by sequence specific primers (SSP) for DR locus and by both SSP and serology for Class I which was reconfirmed on fresh samples. RESULTS: In one patient crossing over had taken place in the region of A locus resulting in inheritance of A*01 instead of expected A*11. In second family crossing over had taken place in region of DRB1 locus and the sibling inherited DRB1*08 instead of DRB1*10. CONCLUSIONS: Possibility of recombination must be considered when interpreting implausible tissue-typing results of families worked up for BMT.enAMLhuman leukocyte antigenrecombinationsequence specific primersAsian Continental Ancestry Group --geneticsFamilyHistocompatibility AntigensHLA Antigens --geneticsFamilyHLA Antigens --geneticsHistocompatibility AntigensHumansIndiaMajor Histocompatibility ComplexRecombination, GeneticArticle