Zaidi, S I MPandey, R NKidwai, A MMurti, C R Krishna2015-07-202015-07-201981-09Zaidi S I M, Pandey R N, Kidwai A M, Murti C R Krishna. A rapid method for preparation of sarcolemma from frog skeletal muscle. Journal of Biosciences. 1981 Sept; 3(3): 293-302.http://imsear.searo.who.int/handle/123456789/160160A rapid method for the preparation of sarcolema from frog skeletal muscle has been described. The purified cell segments were transparent and devoid of contractile material. The Na+, Κ+ -ATPase and 5'-nucleotidase activities in sarcolemma purified by this method were comparable to those reported for sarcolemmal preparations purified by density gradient centrifugation. The preparation also possessed acid phosphatase, alkaline phosphatase and Κ+-activated, ouabain-sensitive p-nitrophenyl phosphatase activities. The cholesterol to phospholipid ratio of the sarcolemma was 0.33, indicating its high purity; further, the preparation was free from mitochondria and contractile proteins.enSkeletal muscleplasma membranesarcolemmaArticle