Chanawong, AroonwadeeLulitanond, AroonlugKaewkes, WanlopLulitanond, ViraphongSrigulbutr, SukanyaHomchampa, Preecha2009-05-272009-05-272007-05-20Chanawong A, Lulitanond A, Kaewkes W, Lulitanond V, Srigulbutr S, Homchampa P. CTX-M extended-spectrum beta-lactamases among clinical isolates of Enterobacteriaceae in a Thai university hospital. The Southeast Asian Journal of Tropical Medicine and Public Health. 2007 May; 38(3): 493-500http://imsear.searo.who.int/handle/123456789/31566The Southeast Asian Journal of Tropical Medicine and Public Health.This study presents updates on molecular epidemiology of extended-spectrum beta-lactamases (ESBLs) in clinical isolates of Enterobacteriaceae from Srinagarind Hospital, Khon Kaen University, Thailand. All isolates were screened for the presence of ESBL genes, bla(TEM), bla(SHV), bla(VEB) and bla(CTX-M), using PCR followed by nucleotide sequence determination. The results revealed that beta-lactamase genes among 48 isolates collected between 1998 and 1999 were bla(SHV) (79%), bla(CTX-M-9) (52%), bla(TEM-1) (48%) and bla(VEB) (33%), whereas those found in 52 isolates collected in 2003 were bla(TEM-1) (79%), bla(CTX-M-15) (44%), bla(SHV) (36%), bla(VEB) (36%), bla(CTX -M-14) (11%) and bla(CTX-M-9) (10%). In addition, 45 isolates carried at least two different ESBL genes. Using PCR, part of insertion sequence ISEcpl was found in the upstream regions of bla(CTX-M-14) and bla(CTX-M-15). ERIC-PCR analysis revealed that most ESBL-producing isolates were of different strains. This is the first report of CTX-M-9, CTX-M-14 and CTX-M-15 beta-lactamase genes in Enterobacteriaceae in Thailand.engAnti-Bacterial AgentsCephalosporins --therapeutic useEnterobacteriaceae --geneticsEpidemiology, MolecularGenetic ScreeningHospitals, UniversityHumansPolymerase Chain ReactionThailandbeta-Lactamases --geneticsJournal Article