Tay, S THabsah, M YTan, S CRohani, M Y2009-05-272009-05-272000-12-21Tay ST, Habsah MY, Tan SC, Rohani MY. Isolation and polymerase chain reaction detection of Mycoplasma pneumoniae in Malaysian patients with respiratory tract infections. The Southeast Asian Journal of Tropical Medicine and Public Health. 2000 Dec; 31(4): 688-92http://imsear.searo.who.int/handle/123456789/33169The Southeast Asian Journal of Tropical Medicine and Public Health.Isolation and polymerase chain reaction (PCR) were performed for detection of Mycoplasma pneumoniae from respiratory tract specimens obtained from 200 adult and 200 pediatric patients. M. pneumoniae was isolated from bronchoalveolar lavage fluid of 1(0.5%) adult patient and 4(2.0%) tracheal aspirates of pediatric patients. PCR was positive for only one (0.5%) broncoalveolar lavage fluid of an adult patient and fifteen (7.5%) tracheal aspirates of pediatric patients. This study suggested that M. pneumoniae was more frequently detected in pediatric patients and PCR appears to have advantages over isolation, in terms of rapidity and sensitivity.engAdultBase SequenceChildChild, PreschoolDNA PrimersFemaleHumansMalaysia --epidemiologyMaleMycoplasma Infections --epidemiologyMycoplasma pneumoniae --geneticsPolymerase Chain ReactionRespiratory Tract Infections --epidemiologyJournal Article