Barde, Pradip VShukla, Mohan KBharti, Praveen KKori, Bhupesh KJatav, Jayant KSingh, Neeru2014-06-182014-06-182014-01WHO South-East Asia Journal of Public Health 2014;3(1): 36-40.http://imsear.searo.who.int/handle/123456789/152141Background: Dengue and chikungunya present with very similar signs and symptoms in the initial stage of illness and so it is difficult to distinguish them clinically. Both are transmitted by Aedes aegypti and Aedes albopictus mosquitoes. This study was conducted with the aim to explore the co-circulation of dengue and chikungunya viruses in central India. Materials and methods: Samples from suspected dengue cases were subjected to dengue immunoglobulin M (IgM) enzyme-linked immunosorbent assay (ELISA) and dengue-negative samples were tested with chikungunya-specific IgM ELISA. The samples collected in acute phase of illness were tested by nested reverse transcription polymerase chain reaction (nRT-PCR). Chikungunya virus (CHIKV) sequences were analysed to determine their genotype. Results: Of 138 samples screened for dengue, 21 (15.2%) were positive, and of 119 samples screened for chikungunya, 13 (10.9%) were positive. Dengue viruses 1 and 4 were found co-circulating with chikungunya virus in Jabalpur, central India. The chikungunya virus detected belonged to the East Central South African genotype. Conclusion: Accurate and timely diagnosis would help in patient management and use of resources. It is advocated to simultaneously test samples for these two diseases in endemic areas. This will also aid in understanding the epidemiology of chikungunya.enWHO Regional Office for South-East AsiaCentral Indiachikungunyaco-circulationdengueArticle