Wangkheimayum, JBhattacharjee, MDas, BJSingha, K MChanda, DDChanda, DDBhowmik, DBhattacharjee, A2020-04-232020-04-232019-09Wangkheimayum J, Bhattacharjee M, Das BJ, Singha K M, Chanda DD, Chanda DD, Bhowmik D, Bhattacharjee A. Diverse aminoglycoside phosphotransferase types conferring aminoglycoside resistance in Enterobacteriaceae: A single-centre study from Northeast India. Indian Journal of Medical Microbiology. 2019 Sep; 37(3): 418-4220255-08571998-3646http://imsear.searo.who.int/handle/123456789/198895The present study investigates the molecular basis of aph-mediated aminoglycoside resistance and their transmission dynamics in a tertiary care hospital of Northeast India. Two hundred forty one isolates (230 Escherichia coli and 11 Klebsiella pneumoniae) were collected and screened for aminoglycoside resistance genes. Various aph types were amplified using polymerase chain reaction (PCR) assay. Plasmid incompatibilty, horizontal transferability and ERIC-PCR based typing were carried out for all the positive isolates. Among them, 67 isolates showed the presence of aph gene. Aph (3“)-IIIa and aph (3')-Via were predominant and horizontally transferable. All the plasmids were of incompatibility I1 group. Twenty-eight different haplotypes of E. coli were found harbouring aph gene types. This study was able to identify diverse aph types in a single centre and their corresponding phenotypic trait.Aminoglycoside resistanceantibiotic resistanceaminoglycoside phosphotransferaseEscherichia coliJournal ArticleIndiaDepartment of Microbiology, Assam University, Silchar, Assam, IndiaDepartment of Microbiology, Silchar Medical College and Hospital, Silchar, Assam, India