Abraham, AmithPhilip, ShajiNarayanan, Sunilkumar PJacob, Kuruvilla CSindhu, RaveendranPandey, AshokSang, Byoung-InJayachandran, Kochupurackal2020-01-022020-01-022019-11Abraham Amith, Philip Shaji, Narayanan Sunilkumar P, Jacob Kuruvilla C, Sindhu Raveendran, Pandey Ashok, Sang Byoung-In, Jayachandran Kochupurackal. A novel microwave mediated disinfection method for hospital linen. Indian Journal of Experimental Biology. 2019 Nov; 57(11): 806-8120975-10090019-5189http://imsear.searo.who.int/handle/123456789/191525Abnormal leaf fall (ALF) disease caused by Phytophthora meadii McRae is one of the limiting factors of growth and latex yield in Hevea brasiliensis (Willd. ex A.Juss.) Müll.Arg. Endophytic bacteria residing within the host plant offer very high application potential as biocontrol agents for ALF disease management. Screening of bacterial endophytes from leaf, petiole and root tissues of Hevea brasiliensis was done for the selection of a potent antagonistic isolate against P. meadii. The most efficient antagonistic isolate was identified as Alcaligenes sp. The biochemical estimation of antagonist treated plants showed induction of peroxidase activity in leaves. Quantitative PCR analysis confirmed higher activity of lignin biosynthesis promoting class III peroxidase transcripts in antagonist treated plants of H. brasiliensis. Homology model of the peroxidase class III protein was built from the deduced amino acid sequence and was found to carry 36% helical and 5% beta sheets. Computer aided docking studies of the substrates (p-coumaryl, coniferyl and sinapyl alcohol) with the target proteins showed that the protein-substrate complexes were stabilized by various interactions and proposed that precursors of lignin biosynthesis were preferred by induced peroxidase in Hevea brasiliensis for lignifications. The present study revealed the potential of an antagonistic endophyte Alcaligenes sp. EIL-2 for inducing class III peroxidase involved in defence mechanism in Hevea brasiliensis.Journal ArticleIndiaRubber Research Institute of India, Kottayam, Kerala, IndiaDepartment of Chemical Engineering, Hanyang University, 222 Wangshimni-ro, Seongdong-gu, Seoul 04763, Republic of KoreaInstitute of Biophysics and Physical Biochemistry, Centre of Magnetic Resonance in Chemistry and Biomedicine (CMRCB) University of Regensburg, D-93040 Regensburg, GermanyMicrobial Processes and Technology Division, CSIR-National Institute for Interdisciplinary Science and Technology (CSIR-NIIST), Thiruvananthapuram-695 019, Kerala, India.Centre for Innovation and Translational Research, CSIR-Indian Institute of Toxicology Research (IITR), 31 MG Marg, Lucknow-226 001, Uttar Pradesh, IndiaSchool of Biosciences, Mahatma Gandhi University, Kottayam, Kerala, India