Fu, YaruLi, ShaokeGuo, QiqiangZheng, WeilieYang, RuiLi, Huie2020-11-182020-11-182019-02Fu Yaru, Li Shaoke, Guo Qiqiang, Zheng Weilie, Yang Rui, Li Huie. Genetic diversity and population structure of two endemic Cupressus (Cupressaceae) species on the Qinghai-Tibetan plateau. Journal of Genetics. 2019 Feb; 98: 1-100022-13330973-7731http://imsear.searo.who.int/handle/123456789/215372Cupressus gigantea and C. torulosa are ecologically and economically important endemic species of the conifer family Cupressaceae on the Qinghai-Tibetan plateau. C. gigantea was previously classified as a subspecies of C. torulosa because of their similar morphological characteristics and close distribution. In this study, 401 individuals were sampled from 16 populations of the two Cupressus species. The specimens were genotyped using 10 polymorphic microsatellite loci through fluorescence polymerase chain reaction (PCR). The genetic diversity of C. gigantea and C. torulosa populations was generally low, with the highest genetic diversity detected in the population LLS of C. gigantea. Distance-based phylogenetic and principal co-ordinates analyses indicated a clear genetic structures for the 16 populations of the two Cupressus species. Moreover, Mantel test results showed indistinctive correlations between population-pairwise Fst values and geographic distances, as well as between genetic distances and geographic distances in C. gigantea and C. torulosa, respectively. AMOVA suggested that genetic variation mostly resided within populations. Sixteen naturalpopulations were evidently clustered into two major groups in the constructed neighbour-joining tree. The results demonstrated that C. gigantea and C. torulosa are different Cupressus species. The genetic information provided important theoretical references for conservation and management of the two endangered Cupressus species.genetic diversitygenetic structuremicrosatelliteCupressus giganteaCupressus torulosaJournal ArticleIndiaTibet Agricultural and Animal Husbandry College, Nyingchi 860000, Tibet, People’s Republic of ChinaKey Laboratory of Forest Ecology in Tibet Plateau, Ministry of Education, Nyingchi 860000, Tibet, People’s Republic of ChinaNational Key Station for Field Scientific Observation and Experiment, Nyingchi 860000, Tibet, People’s Republic of ChinaTibet Agricultural and Animal Husbandry College, Nyingchi 860000, Tibet, People’s Republic of ChinaKey Laboratory of Forest Ecology in Tibet Plateau, Ministry of Education, Nyingchi 860000, Tibet, People’s Republic of ChinaNational Key Station for Field Scientific Observation and Experiment, Nyingchi 860000, Tibet, People’s Republic of ChinaTibet Agricultural and Animal Husbandry College, Nyingchi 860000, Tibet, People’s Republic of ChinaKey Laboratory of Forest Ecology in Tibet Plateau, Ministry of Education, Nyingchi 860000, Tibet, People’s Republic of ChinaNational Key Station for Field Scientific Observation and Experiment, Nyingchi 860000, Tibet, People’s Republic of ChinaCollege of Forestry, Guizhou University, Guiyang 550025, Guizhou, People’s Republic of ChinaCollege of Agriculture, Guizhou University, Guiyang 550025, Guizhou, People’s Republic of China