Hajela, KKayestha, RSumati,1996-08-012009-05-271996-08-012009-05-271996-08-01Hajela K, Kayestha R, Sumati . Carbohydrate induced modulation of cell membrane. IV: Interaction with mucin and fucoidan totally immobilizes the human platelet membrane. Indian Journal of Biochemistry & Biophysics. 1996 Aug; 33(4): 308-10http://imsear.searo.who.int/handle/123456789/27467P selectins and E selectins are cell adhesion molecules that mediate the interaction of platelets and endothelial cells with neutrophils and monocytes. The proposed ligands for these receptors contain the Le(x) core, sialic acid and sulfated fucose. In this paper we report that binding of sialic acid markedly restricts the mobility of membrane proteins and lipids as studied by EPR spectroscopy using spin probes. Binding of mucin and fucoidan totally restricts the mobility probably due to cross-linking of the surface lectins. Binding of these ligands also resulted in an increase in the cytoplasmic viscosity.engBlood Platelets --chemistryCell Membrane --chemistryElectron Spin Resonance SpectroscopyHumansLigandsMembrane Lipids --chemistryMembrane Proteins --chemistryMucins --pharmacologyPolysaccharides --pharmacologySelectins --metabolismIn Vitro