Singh, VBagga, PKSingh, BJaideep2020-09-242020-09-242020-02Singh V, Bagga PK, Singh B, Jaideep. Application of Thyroid Peroxidase (TPO) and Hector Battifora Mesothelial-1 (HBME-1) immunohistochemical markers in the diagnosis of papillary thyroid carcinoma of the thyroid. International Journal of Medical Research & Review. 2020 Feb; 8(1): 110-1172320-86862321-127Xhttp://imsear.searo.who.int/handle/123456789/205331Background: Thyroid cancer is the most common endocrine malignancy accounting for >90% of malignancies of endocrine glands. The inter and intraobserver variation in the histomorphological diagnosis of Papillary Thyroid Carcinomas may sometimes pose a diagnostic difficulty. Application of IHC biomarkers may play an active or complementary role in their accurate classification. Aim: The present study was conducted to evaluate if HBME-1 and TPO immunohistochemical analysis can reliably differentiate papillary carcinomas from other thyroid lesions. Material and Methods: 50 cases of benign and malignant thyroid lesions were taken. Immunohistochemical staining for HBME-1 and TPO was performed. HBME-1 and TPO score was interpreted as absent and positive. Medical records were retrieved and their clinical data, surgical treatment, and pathological findings were noted. Results: Out of 50 cases, 19 (73.1%) cases were diagnosed PTC, 4 (15.4%) cases were FTC, 3(11.5%) cases were of MTC and 24 cases of benign thyroid lesions. TPO expression was found positive in 91.7% of cases of Benign thyroid lesions. In malignant thyroid lesions, negative expression of TPO was seen in 63.16%, 0% and 33.33% of PTC, FCT, and MCT respectively. HBME-1 showed negative expression in 83.3% of cases of benign thyroid lesions. Whereas, in malignant thyroid lesion HBME-1 expression was positive in 78.95%, 50% and 0% cases of PTC, FCT, and MCT respectively. Conclusion: Testing for expression of HBME-1 has been shown to improve the diagnostic accuracy for thyroid malignant nodules. The combination of HBME-1, and TPO may contribute to an accurate diagnosis of papillary thyroid carcinoma.TPOHBME-1Papillary Thyroid CarcinomaImmunohistochemistryJournal ArticleIndiaDr. Vasundhara Singh Junior Resident, Department of Pathology, Government Medical College, Amritsar, Punjab, IndiaDr. Permeet Kaur Bagga Associate Professor, Department of Pathology, Government Medical College, Amritsar, Punjab, IndiaDr. Bikramjeet Singh Associate Professor, Department of Surgery, Government Medical College, Amritsar, Punjab, IndiaDr Jaideep Assistant Professor, Department of Pathology, Government Medical College, Amritsar, Punjab, India