Wang, YicunGao, Xianggang, JU ZhiLiu, JingyuanDong, ShuaiWang, Li2015-02-092015-02-092013-07Wang Yicun, Gao Xiang, gang JU Zhi, Liu Jingyuan, Dong Shuai, Wang Li. Detection of Candida albicans Sap2 in cancer patient serum samples by an indirect competitive enzyme-linked immunosorbent assay for the diagnosis of candidiasis. Indian Journal of Pathology & Microbiology. 2013 Jul-Sept 56 (3): 243-247.http://imsear.searo.who.int/handle/123456789/155876Background: The secreted aspartyl proteinases 2 (Sap2) of Candida albicans (C. albicans) is a potential marker of candididasis. It is a virulence factor associated with adherence and tissue invasion. Aim: In order to detect Sap2 in clinical sera, we developed an indirect competitive enzyme-linked immunosorbent assay (ELISA). Materials and Methods: Polyclonal antibodies were produced for Sap2 by injecting Sap2 into a New Zealand White inbred rabbit. They could be used at a dilution exceeding 1:1200 in an indirect ELISA, and detected Sap2 concentration up to 1 ng/mL. Results: Of the 286 cancer serum samples tested, 16.8% were found as candidiasis. The test was simple and economical to perform and had a level of sensitivity for detection of low-titer positive sera; thus, it may be proven to be of value in epidemiological studies on candidiasis.enAntibodycancerCandida albicansELISASap2Article