Felice, Francesca DiMicheli, GioacchinoCamilloni, Giorgio2020-11-182020-11-182019-06Felice Francesca Di, Micheli Gioacchino, Camilloni Giorgio. Restriction enzymes and their use in molecular biology: An overview. Journal of Biosciences. 2019 Jun; 44(2): 1-80250-59910973-7138http://imsear.searo.who.int/handle/123456789/214377Restriction enzymes have been identified in the early 1950s of the past century and have quickly become key players in themolecular biology of DNA. Forty years ago, the scientists whose pioneering work had explored the activity and sequencespecificity of these enzymes, contributing to the definition of their enormous potential as tools for DNA characterization,mapping and manipulation, were awarded the Nobel Prize. In this short review, we celebrate the history of these enzymes inthe light of their many different uses, as these proteins have accompanied the history of DNA for over 50 years representingactive witnesses of major steps in the fieldCloningDNA manipulationhistoryrestriction enzymesJournal ArticleIndiaDipartimento di Biologia e Biotecnologie, Sapienza, Universita` di Roma, Piazzale A. Moro 5, 00185 Roma, ItalyIstituto di Biologia e Patologia Molecolari, CNR, Roma, Piazzale A. Moro 5, 00185 Roma, ItalyDipartimento di Biologia e Biotecnologie, Sapienza, Universita` di Roma, Piazzale A. Moro 5, 00185 Roma, Italy Istituto di Biologia e Patologia Molecolari, CNR, Roma, Piazzale A. Moro 5, 00185 Roma, Italy