Rao, B N NarasingaRamakrishnan, CBalaram, P2015-07-172015-07-171979-03Rao B N Narasinga, Ramakrishnan C, Balaram P. Metal binding to pyridoxal derivatives. An NMR study of the interaction of Eu(III) with pyridoxal phosphate and pyridoxamine phosphate. Journal of Biosciences. 1979 Mar; 1(1): 35-47.http://imsear.searo.who.int/handle/123456789/159922The solution conformations of pyridoxal-5'-phosphate and pyridoxamine- 5'-phosphate have been investigated using Eu(III) as a nuclear magnetic resonance shift probe. Binding of Eu(III) to pyridoxal phosphate results in the formation of two complexes, at the phosphate group and the o-hydroxy-aldehyde moiety, which are in slow exchange on the nuclear magnetic resonance time-scale. The lanthanide-induced pseudo contact shifts calculated using the McConnell-Robertson equation (J. Chem. Soc. (1950), 22, 1561) are in good agreement with the experimentally observed values for both pyridoxal phosphate and pyridoxamine phosphate and lead to a family of closely related conformations.enConformationlanthanidenuclear magnetic resonancepyridoxal-5' phosphatepyridoxamine-5'-phosphateArticle