Vincent, Mary GraceJohn, Neetu PeedikayilNarayanan, P MVani, V CMurugan, Sevanan2015-07-022015-07-022014-07Vincent Mary Grace, John Neetu Peedikayil, Narayanan P M, Vani V C, Murugan Sevanan. In vitro study on the efficacy of zinc oxide and titanium dioxide nanoparticles against metallo beta-lactamase and biofilm producing Pseudomonas aeruginosa. Journal of Applied Pharmaceutical Science. 2014 July; 4(7): 41-46.2231-3354http://imsear.searo.who.int/handle/123456789/158865Pseudomonas aeruginosa contributes to a multitude of infections exhibiting intrinsic resistance to numerous antibiotics. Metallo beta-lactamase and biofilm production is the most worrisome resistant mechanisms observed in P. aeruginosa. Emergence of antimicrobial resistance by pathogenic bacteria is a major health problem in recent years. Zinc oxide and titanium dioxide nanoparticles comprises of well-known inhibitory and bactericidal effects. The present study is designed to determine the efficacy of zinc oxide and titanium dioxide nanoparticles against metallo beta-lactamase and biofilm producing P. aeruginosa. A total of 51 non-repetitive P. aeruginosa isolates were obtained from Bioline laboratory, Coimbatore. Biofilm and metallo beta-lactamase production was tested by combined disc test and tissue culture plate method. Commercially available zinc and titanium dioxide nanoparticles were obtained and tested against two metallo beta-lactamase and biofilm producing isolates. Both the nanoparticles showed appreciable activity at all tested concentrations. Thus, it is concluded that ZnO and TiO2 nanoparticles may serve as a promising antibacterial agents in coming years.enPseudomonas aeruginosaBiofilmsMetallo betalactamaseZinc oxide nanoparticlesTitanium dioxide nanoparticlesAntimicrobial activityArticle