Sen, PrakritiGanguly, PoojaKulkarni, Kirti KBudhwar, RoliGanguly, Niladri2020-11-182020-11-182020-08Sen Prakriti, Ganguly Pooja, Kulkarni Kirti K, Budhwar Roli, Ganguly Niladri. Differential transcriptome analysis in HPV-positive and HPV-negative cervical cancer cells through CRISPR knockout of miR-214. Journal of Biosciences. 2020 Aug; : 1-100250-59910973-7138http://imsear.searo.who.int/handle/123456789/214253In this study we have investigated the effects of a tumour suppressor microRNA, miR-214, on gene expressionin HPV-positive (CaSki) and HPV-negative cervical cancer cells (C33A) by RNA sequencing using nextgeneration sequencing. The HPV-positive and HPV-negative cervical cancer cells were either miR-214-knocked-out or miR-214-overexpressed. Gene expression analysis showed that a total of 904 genes wereupregulated and 365 genes were downregulated between HPV-positive and HPV-negative cervical cancer cellswith a fold change of ±2. Furthermore, 11 differentially expressed and relevant genes (TNFAIP3, RAB25,MET, CYP1B1, NDRG1, CD24, LOXL2, CD44, PMS2, LATS1 and MDM1) which showed a fold change of ±5were selected to confirm by real-time PCR. This study represents the first report of miR-214 on global geneexpression in the context of HPV.Cervical cancerCRISPRHuman papilloma virusmiR-214RNA transcriptomeJournal ArticleIndiaCancer Biology Lab, School of Biotechnology, KIIT University, Bhubaneswar, IndiaCancer Biology Lab, School of Biotechnology, KIIT University, Bhubaneswar, IndiaBionivid Technology Pvt. Ltd., Kasturi Nagar, Bengaluru, IndiaBionivid Technology Pvt. Ltd., Kasturi Nagar, Bengaluru, IndiaCancer Biology Lab, School of Biotechnology, KIIT University, Bhubaneswar, India