Colognese, MRVCardoso, PM de FFurlan, JTUeda, JKCamilotti, V.2024-11-302024-11-302024-08Colognese MRV, Cardoso PM de F, Furlan JT, Ueda JK, Camilotti V.. Evaluation of Bond Strength of a Bioactive Adhesive System Associated with Ozonized Water on Enamel Preconditioned with Phosphoric Acid. Journal of Advances in Medicine and Medical Research. 2024 Aug; 36(9): 63-692456-8899https://imsear.searo.who.int/handle/123456789/237250Aims: To evaluate the effectiveness of ozonated water as a cavity cleaning solution in combination with a bioactive adhesive system on bond strength to enamel, compared to distilled water, after different storage times. Study Design: Experimental in vitro study. Place and Duration of Study: Department of Restorative Dentistry, State University of West Parana, Cascavel, Parana, Brazil, between September 2022 and August 2023. Methodology: Bovine anterior teeth were divided into four groups: AD24h (distilled water and 24-hour storage), AO24h (ozonated water and 24-hour storage), AD30D (distilled water and 30-day storage), and AO30D (ozonated water and 30-day storage). After cavity cleaning, a bioactive adhesive system and composite resin were applied. Three bioactive composite resin cylinders were light-cured on each tooth using a LED light device. Microshear bond strength was tested with a testing machine (EMIC) at a speed of 1 mm/min using a 50N load cell. Statistical analysis was performed using the non-parametric Wilcoxon test (p < 0.05). Results: Ozonated water significantly increased bond strength to enamel after 24 hours (p < 0.05). However, after 30 days, no significant differences in bond strength were observed between ozonated water and distilled water groups. Conclusion: Ozonated water enhances initial bond strength to enamel but its effect diminishes over time. These findings suggest the need for improved adhesion techniques to achieve long-term durability in dental restorations.Bond strengthbioactive adhesive systemozonated wateracid conditioningmicroshear bond strengthdental restorationsadhesion durabilityJournal ArticleIndiaDepartment of Restorative Dentistry, School of Dentistry, State University of West Parana, Cascavel, Parana, BrazilDepartment of Restorative Dentistry, School of Dentistry, State University of West Parana, Cascavel, Parana, BrazilDepartment of Restorative Dentistry, School of Dentistry, State University of West Parana, Cascavel, Parana, BrazilDepartment of Restorative Dentistry, School of Dentistry, State University of West Parana, Cascavel, Parana, BrazilDepartment of Restorative Dentistry, School of Dentistry, State University of West Parana, Cascavel, Parana, Brazil