Kumar, DDhiman, SRabha, BGoswami, DYadav, KVeer, VBaruah, I2016-08-102016-08-102016-04Kumar D, Dhiman S, Rabha B, Goswami D, Yadav K, Deka M, Veer V, Baruah I. Typing of Plasmodium falciparum DNA from 2 years old Giemsa‑stained dried blood spots using nested polymerase chain reaction assay. Indian Journal of Medical Microbiology. 2016 Apr-June; 34(2): 210-212.http://imsear.searo.who.int/handle/123456789/176590A panel of 129 Giemsa‑stained thick blood spots (TBS) confirmed for Plasmodium falciparum infection having different levels of parasite density were collected from a malaria endemic area. DNA was extracted and nested polymerase chain reaction (PCR) assay was performed to amplify P. falciparum DNA. Nested PCR assay successfully amplified P. falciparum DNA at a very low parasitaemia of ~10 parasites/μl of blood. Current PCR assay is very simple and can be used retrospectively to monitor the invasion and prevalence of different Plasmodium species in endemic areas.enParasitaemiaPlasmodiumpolymerase chain reactionthick blood spotsArticle