Ren, JianguoWang, JunliKarthikeyan, SivakumaranLiu, HongmeiCai, Jing2020-01-022020-01-022019-04Ren Jianguo, Wang Junli, Karthikeyan Sivakumaran, Liu Hongmei, Cai Jing. Natural anti-phytopathogenic fungi compound phenol, 2, 4-bis (1, 1-dimethylethyl) from Pseudomonas fluorescens TL-1. Indian Journal of Biochemistry and Biophysics. 2019 Apr; 56(2): 162-1680975-09590301-1208http://imsear.searo.who.int/handle/123456789/190040A strain was isolated from tobacco phylloplane and preliminarily identified as Pseudomonas fluorescens TL-1, which had the visible inhibition against ten plant pathogenic fungi, viz., Curvularia lunata, Bipolaris maydis, Valsa mali, Rhizoctonia solani, Colletotrichum gloeosporioides, Sclerotinia sclerotiorum, Botrytis cinerea, Alternaria alternate, Fusarium oxysporum and Verticillium dahlia in dual culture experiments. The ethyl acetate extract of nutrient broth seeded with Pseudomonas fluorescens TL-1 suspension was separated into fifty-nine fractions by the Sephadex LH-20 column and the antifungal activity of each fraction was tested with paper disc diffusion method against Curvularia lunata. The results showed that fraction 1 to 3 had the strongest inhibitory effects on Curvularia lunata. Furthermore, GC/MS analysis of the constituents of fraction 1 to 59 confirmed that phenol, 2, 4-bis (1, 1-dimethylethyl) was the active compound for the antifungal activity from Pseudomonas fluorescens TL-1.Journal ArticleIndiaCenter of Research and Development of Fine Chemicals, Guizhou UniversitySchool of Biology and Engineering, Guizhou Medical UniversitySchool of Public Health/Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guiyang -550 025, Guizhou, ChinaDepartment of Physics, Dr. Ambedkar Government Arts College, Chennai -600 039, Tamil Nadu, India