Manobala, TShukla, Sudhir KRao, T SubbaKumar, M Dharmendira2020-11-182020-11-182019-10Manobala T, Shukla Sudhir K, Rao T Subba, Kumar M Dharmendira. A new uranium bioremediation approach using radio-tolerant Deinococcus radiodurans biofilm. Journal of Biosciences. 2019 Oct; 44(5): 1-90250-59910973-7138http://imsear.searo.who.int/handle/123456789/214187Deinococcus radiodurans is the most radiation-tolerant organism ever known. It has gained importance in recent years as apotential candidate for bioremediation of heavy metals, especially the radioactive type. This study investigates the efficiency of a recombinant D. radiodurans (DR1-bf?) strain with an ability to form biofilm for uranium remediation. Themodified Arsenazo III dye method was used to estimate the uranium concentration. Uranyl nitrate aqueous solution wasgenerated during the operation of nuclear fuel reprocessing. The D. radiodurans biofilm (DR1-bf?) grown in the presenceof 20 mM Ca2? showed remarkable ability of uranyl ion removal. DR1-bf? (?Ca2?) biofilm removed *75±2% of1000 mg/L uranium within 30 min post-treatment from uranyl nitrate aqueous solution. Uranium removal rate was alsofound to be directly proportional to biofilm age. This study discusses the ability of D. radiodurans biofilm in uraniumremoval.ArsenazobiofilmbioremediationDeinococcus radioduransradio-toleranceuraniumJournal ArticleIndiaDepartment of Applied Science and Technology, Anna University, Chennai 600 025, IndiaBiofouling and Thermal Ecology Section, Water and Steam Chemistry Division, BARC Facilities, Kalpakkam 603 102, IndiaBiofouling and Thermal Ecology Section, Water and Steam Chemistry Division, BARC Facilities, Kalpakkam 603 102, IndiaDepartment of Applied Science and Technology, Anna University, Chennai 600 025, India