Kulkarni, Shrinivas KPatil, Chandrashekhar SJain, Naveen KSingh, Amarjit2009-05-282009-05-282004-06-21Kulkarni SK, Patil CS, Jain NK, Singh A. Modulatory effect of diclofenac on antispasmodic effect of pitofenone in cholinergic spasm. Indian Journal of Experimental Biology. 2004 Jun; 42(6): 567-9http://imsear.searo.who.int/handle/123456789/63185Biliary, ureteric and intestinal colic are extremely common clinical conditions associated with smooth muscle spasm. In the present study, antispasmodic activity was carried out against acetylcholine (10-640 ng/ml)-induced contractions on guinea pig ileum. Acetylcholine (10-640 ng/ml) induced concentration-dependent contraction of smooth muscle. Diclofenac, in varying concentration (9.4 x 10(-5) mol/l and 14.1 x 10(-5) mol/l) shifted the concentration response curve of acetylcholine to the right without suppressing the maximal response. However, in higher concentration diclofenac (18.9 x 10(-5) mol/l) blocked the response in an unsurmountable fashion. Further, analgin (11.09 x 10(-5), 16.63 x 10(-5) and 22.18 x 10(-5) mol/l) in equimolar concentrations did not alter the concentration response curve of acetylcholine, but in higher concentration analgin (44.36 x 10(-5) mol/l) also blocked the response in an unsurmountable fashion. Pitofenone (2.5 x 10(-6) mol/l) also, shifted the concentration response curve of acetylcholine to right in a parallel fashion with no change in maximal response. The present study confirms the potent antispasmodic activity of diclofenac-pitofenone combination in comparison to analgin-pitofenone in molar equivalent concentration (in comparison to diclofenac) against acetylcholine-induced contractions of guinea pig ileum.engAcetylcholine --pharmacologyAnimalsAnti-Inflammatory Agents, Non-Steroidal --pharmacologyBenzophenones --pharmacologyCholinergic Agents --metabolismDiclofenac --pharmacologyDipyrone --pharmacologyDose-Response Relationship, DrugFemaleGuinea PigsIleum --drug effectsMaleMuscle ContractionMuscle, Smooth --drug effectsParasympatholytics --pharmacologySpasm --drug therapyJournal Article