Lintag, I CKobayashi, NUrasawa, TTaniguchi, KSaniel, M CUrasawa, S2009-05-272009-05-271990-06-01Lintag IC, Kobayashi N, Urasawa T, Taniguchi K, Saniel MC, Urasawa S. Determination of subgroup, serotype and electropherotype of human rotaviruses obtained from diarrheic patients in the Philippines. The Southeast Asian Journal of Tropical Medicine and Public Health. 1990 Jun; 21(2): 249-58http://imsear.searo.who.int/handle/123456789/35837The Southeast Asian Journal of Tropical Medicine and Public Health.A total of 40 rotavirus specimens obtained from infants with acute gastroenteritis in Metro Manila, Philippines, were analyzed for their subgroup and serotype specificities and RNA electropherotype. A half (50%) of the specimens contained subgroup I human rotaviruses (HRVs), while 45% of them contained subgroup II HRVs. All subgroup II HRVs were determined to be serotype 1, except one specimen belonging to serotype 3, by an enzyme-linked immunosorbent assay with serotype specific monoclonal antibodies. However, serotype specificity of all subgroup I specimens failed to be determined. In polyacrylamide gel electrophoresis, RNAs extracted from HRVs of either subgroup showed long electropherotype. At least two electropherotypes were distinguished in subgroup I viruses, while five different patterns of viral RNA were found in subgroup II viruses.engChild, PreschoolElectrophoresis, Gel, Two-DimensionalEnzyme-Linked Immunosorbent AssayFemaleGastroenteritis --epidemiologyHumansInfantInfant, NewbornMalePhilippines --epidemiologyRNA --analysisRotavirus --classificationRotavirus Infections --epidemiologySeroepidemiologic StudiesSerotypingJournal Article