Devi, Manju SSudhakaran, Perumana R2012-08-092012-08-092012-02Devi Manju S, Sudhakaran Perumana R. Angiogenic response of advanced glycation end products (AGEs) involves PPAR .Indian Journal of Biochemistry & Biophysics. 2012 Feb; 49(1): 18-24.http://imsear.searo.who.int/handle/123456789/140214Diabetes is associated with increased formation of advanced glycation end products (AGEs), which have been implicated in micro and macrovascular complications of diabetes. Our earlier reports showed proangiogenic effect of AGE-bovine serum albumin (BSA). In order to understand the mechanism of AGE-mediated angiogenesis, the possibility of involvement of peroxisome prolifeator activated receptor (PPAR) , a ligand activated transcription factor was examined. The angiogenic effect was studied in chick chorio allantoic membrane (CAM) and by analyzing angiogenic markers in human umbilical vein endothelial cells (HUVECs) in culture. The involvement of PPAR was investigated using synthetic PPAR agonist GW 1929 and antagonist GW 9662 and by RT-PCR. In CAM assay, PPAR antagonist GW 9662 reversed the AGE-induced effect on vascularity. In HUVECs in culture, GW 9662 reversed the effect of AGE-BSA and decreased the expression of CD 31, E-Selectin and VEGF. RT-PCR analysis showed that treatment with AGE-BSA caused upregulation of PPAR mRNA levels. The reversal of the effect of AGE on angiogenesis by treatment with PPAR antagonists and up-regulation of PPAR gene in HUVECs treated with AGE-BSA suggested the possible involvement of PPAR -dependent downstream pathway in mediating the angiogenic effect of AGE.enAdvanced Glycation End Products (AGE s)PPARAngiogenesisDiabetesVEGFAngiogenesis Inducing Agents --metabolismAnilides --pharmacologyAnimalsAntigens, CD31 --drug effectsAntigens, CD31 --metabolismBenzophenones --pharmacologyCells, CulturedChick EmbryoChorioallantoic Membrane --drug effectsChorioallantoic Membrane --metabolismDiabetes Mellitus --metabolismE-Selectin --metabolismGlycosylation End Products, Advanced --pharmacologyHuman Umbilical Vein Endothelial Cells --metabolismPPAR gamma --agonistsPPAR gamma --antagonists & inhibitorsPPAR gamma --drug effectsPPAR gamma --metabolismRNA --drug effectsRNA --metabolismTyrosine --analogs & derivativesTyrosine --pharmacologyVascular Endothelial Growth Factor A --drug effectsVascular Endothelial Growth Factor A --metabolismArticle